Molecular genetic RH typing of single erythropoietic blast-forming units
| Patient . | RH genotype* . | RHD/RHCE specificities tested† . | Number of BFU-E colonies . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Tested . | Excluded‡ . | Interpretable . | Positive forRHD/RHCEspecificities . | ||||||
| CcDe . | ceonly . | DEe . | eonly . | ||||||
| 2 | CcDdee | C, c, D (exon 7), e | 30 | 0 | 30 | 13 | 17 | nd | 0 |
| 3 | CcDdee | C, c, D (exon 7), e | 18 | 7 | 11 | 8 | 3 | nd | 0 |
| 6 | CcDvardee§ | C, c, D (exon 5), e | 40 | 4 | 36 | 29 | 7 | nd | 0 |
| 7 | ccDdEe | D (exon 7), E, e | 40 | 4 | 36 | nd | nd | 15 | 21 |
| Patient . | RH genotype* . | RHD/RHCE specificities tested† . | Number of BFU-E colonies . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Tested . | Excluded‡ . | Interpretable . | Positive forRHD/RHCEspecificities . | ||||||
| CcDe . | ceonly . | DEe . | eonly . | ||||||
| 2 | CcDdee | C, c, D (exon 7), e | 30 | 0 | 30 | 13 | 17 | nd | 0 |
| 3 | CcDdee | C, c, D (exon 7), e | 18 | 7 | 11 | 8 | 3 | nd | 0 |
| 6 | CcDvardee§ | C, c, D (exon 5), e | 40 | 4 | 36 | 29 | 7 | nd | 0 |
| 7 | ccDdEe | D (exon 7), E, e | 40 | 4 | 36 | nd | nd | 15 | 21 |
BFU-E indicates erythropoietic blast-forming unit; and nd, not determined.
As determined from blood DNA samples.
BFU-E samples of patients 2, 3, and 6 were genotyped by real-time PCR, whereas BFU-E samples of patient 7 were genotyped by PCR-SSP (as detailed in ″Patients, materials, and methods″).
Sample exclusion because of amplification failure of positive control.
Partial D variant (D category IV type 4).