Shared TCR-β CDR3 sequences found in PNH patients by systematic cloning and sequencing
| Sequence ID . | Patients . | TRBV . | TCR-β CDR3 . | TRBJ . | ||
|---|---|---|---|---|---|---|
| Vβ . | nDn . | Jβ . | ||||
| S1 | 7, 9 | 7.9 | CASS | LVGGPEQ | YFGP | 2.7 |
| S2 | 6, 16 | 15 | CATS | RGRTQGLDYG | YTFG | 1.2 |
| S3 | 8, 19 | 30 | CA | WEQVIA | FFGQ | 1.1 |
| S4 | 5, 7, 9 | 15 | CATS | GIAGETQ | FFGP | 2.1 |
| S5 | 5, 7, 9 | 15 | CATS | RVAGETQ | YFGP | 2.5 |
| S5a | 9 | 15 | CATS | RIGGETQ | YFGP | 2.5 |
| S5b | 2 | 15 | CATS | RTAGETQ | YFGP | 2.5 |
| Sequence ID . | Patients . | TRBV . | TCR-β CDR3 . | TRBJ . | ||
|---|---|---|---|---|---|---|
| Vβ . | nDn . | Jβ . | ||||
| S1 | 7, 9 | 7.9 | CASS | LVGGPEQ | YFGP | 2.7 |
| S2 | 6, 16 | 15 | CATS | RGRTQGLDYG | YTFG | 1.2 |
| S3 | 8, 19 | 30 | CA | WEQVIA | FFGQ | 1.1 |
| S4 | 5, 7, 9 | 15 | CATS | GIAGETQ | FFGP | 2.1 |
| S5 | 5, 7, 9 | 15 | CATS | RVAGETQ | YFGP | 2.5 |
| S5a | 9 | 15 | CATS | RIGGETQ | YFGP | 2.5 |
| S5b | 2 | 15 | CATS | RTAGETQ | YFGP | 2.5 |
Identical and similar TCR-β CDR3 sequences identified in different PNH patients by cloning and sequencing of the TCR-β repertoire of CD8+ CD57+ T cells. Nomenclature of Vβ (TRBV) and Jβ (TRBJ) genes is according to the International Immunogenetics Information System.30 Patients are numbered as in Table 1.