Closely related TCR-β CDR3 sequences found within individual patients and in different patients with PNH
| Sequence ID . | Patients . | TRBV . | TCR-β CDR3 . | TRBJ . | ||
|---|---|---|---|---|---|---|
| Vβ . | nDn . | Jβ . | ||||
| S4 | 5,* 7,* 9,* 10† | 15 | CATS | GIA GETQ | FFGP | 2.1 |
| S5 | 5,* 7,* 9,* 14† | 15 | CATS | RVA GETQ | YFGP | 2.5 |
| S5a | 9,* 20† | 15 | CATS | RTG GETQ | YFGP | 2.5 |
| S5b | 2* | 15 | CATS | RTA GETQ | YFGP | 2.5 |
| S5c | 5,† 16† | 15 | CATS | RIA GETQ | YFGP | 2.5 |
| S5d | 3† | 15 | CATS | RVG GETQ | YFGP | 2.5 |
| S5e | 5† | 15 | CATS | REI GETQ | YFGP | 2.5 |
| S5f | 5† | 15 | CATS | RDLAGETQ | YFGP | 2.5 |
| Sequence ID . | Patients . | TRBV . | TCR-β CDR3 . | TRBJ . | ||
|---|---|---|---|---|---|---|
| Vβ . | nDn . | Jβ . | ||||
| S4 | 5,* 7,* 9,* 10† | 15 | CATS | GIA GETQ | FFGP | 2.1 |
| S5 | 5,* 7,* 9,* 14† | 15 | CATS | RVA GETQ | YFGP | 2.5 |
| S5a | 9,* 20† | 15 | CATS | RTG GETQ | YFGP | 2.5 |
| S5b | 2* | 15 | CATS | RTA GETQ | YFGP | 2.5 |
| S5c | 5,† 16† | 15 | CATS | RIA GETQ | YFGP | 2.5 |
| S5d | 3† | 15 | CATS | RVG GETQ | YFGP | 2.5 |
| S5e | 5† | 15 | CATS | REI GETQ | YFGP | 2.5 |
| S5f | 5† | 15 | CATS | RDLAGETQ | YFGP | 2.5 |
Identical and quasi-identical TCR-β CDR3 sequences identified in different PNH patients by (1) “cloning and sequencing”* or (2) by clonotypic-specific PCR.† Nomenclature of Vβ (TRBV) and Jβ (TRBJ) genes is according to the International Immunogenetics Information System.30 Patients are numbered as in Table 1, and sequence ID is as identified in Table 2.
Identical and quasi-identical TCR-β CDR3 sequences identified in different PNH patients by “cloning and sequencing.”
Identical and quasi-identical TCR-β CDR3 sequences identified in different PNH patients by clonotypic-specific PCR.