Resting potential of uninjected oocytes or oocytes expressing wt or mutated hAE1
| Em in mV . | Uninjected . | hAE1 . | L687P . | S731P . | D705Y . | H734R . |
|---|---|---|---|---|---|---|
| NaCl/KCl | −46.1 ± 1.7 (12) | −48.0 ± 1.6 (9) | −44.9 ± 2.7 (6)† | −39.8 ± 3.0 (6)† | −38.8 ± 4.1 (4)† | −42.2 ± 1.9 (14)† |
| KCl | −5.9 ± 1.2 (5)* | −7.1 ± 2.7 (5)* | −24.4 ± 1.9 (5)* | −15.6 ± 2.1 (5)* | −11.1 ± 2.3 (5)* | −12.3 ± 1.5 (5)* |
| NMDGCl | −42.4 ± 2.9 (4) | −47.5 ± 2.4 (5) | −53.0 ± 6.6 (5)* | −51.0 ± 3.8 (10)* | −48.3 ± 3.0 (3)* | −51.2 ± 5.0 (3)* |
| Gluconate | −40.3 ± 3.7 (5)* | −40.1 ± 0.2 (5)* | −46.0 ± 7.0 (5) | −34.3 ± 2.2 (5) | −36.5 ± 1.7 (5) | −42.0 ± 0.8 (5) |
| Em in mV . | Uninjected . | hAE1 . | L687P . | S731P . | D705Y . | H734R . |
|---|---|---|---|---|---|---|
| NaCl/KCl | −46.1 ± 1.7 (12) | −48.0 ± 1.6 (9) | −44.9 ± 2.7 (6)† | −39.8 ± 3.0 (6)† | −38.8 ± 4.1 (4)† | −42.2 ± 1.9 (14)† |
| KCl | −5.9 ± 1.2 (5)* | −7.1 ± 2.7 (5)* | −24.4 ± 1.9 (5)* | −15.6 ± 2.1 (5)* | −11.1 ± 2.3 (5)* | −12.3 ± 1.5 (5)* |
| NMDGCl | −42.4 ± 2.9 (4) | −47.5 ± 2.4 (5) | −53.0 ± 6.6 (5)* | −51.0 ± 3.8 (10)* | −48.3 ± 3.0 (3)* | −51.2 ± 5.0 (3)* |
| Gluconate | −40.3 ± 3.7 (5)* | −40.1 ± 0.2 (5)* | −46.0 ± 7.0 (5) | −34.3 ± 2.2 (5) | −36.5 ± 1.7 (5) | −42.0 ± 0.8 (5) |
Resting membrane potential (Em) was measured in regular MBS (NaCl/KCl) or in NMDGCl, KCl, or gluconate containing MBS (“Electrophysiological measurements”). Data are means (± SEM) of n experiments (number in parentheses), each made with 5 oocytes. Em in NaCl/KCl-containing medium was compared with Em in high K+- or NMDG-containing media for each column.
The means compared differed at P < .05. In NaCl/KCl-containing medium, the Em of oocytes expressing mutant hAE1 was compared with Em of oocytes expressing wt hAE1.
The means compared differed at P < .05.