Comparison of immunoreactivities and avidities of 1F5, Lym-1, and HD39 Abs and Ab/SA conjugates
| . | 1F5 . | 1F5/SA . | Lym-1 . | Lym-1/SA . | HD39 . | HD39/SA . |
|---|---|---|---|---|---|---|
| IR, % | 66.50 | 64.15 | 61.30 | 52.60 | 70.3 | 75.10 |
| Avidity, × 108 M−1* | 0.66 ± 0.08 | 0.72 ± 0.08 | 0.74 ± 0.28 | 0.50 ± 0.12 | 7.75 ± 1.26 | 7.52 ± 1.10 |
| . | 1F5 . | 1F5/SA . | Lym-1 . | Lym-1/SA . | HD39 . | HD39/SA . |
|---|---|---|---|---|---|---|
| IR, % | 66.50 | 64.15 | 61.30 | 52.60 | 70.3 | 75.10 |
| Avidity, × 108 M−1* | 0.66 ± 0.08 | 0.72 ± 0.08 | 0.74 ± 0.28 | 0.50 ± 0.12 | 7.75 ± 1.26 | 7.52 ± 1.10 |
Using standard cell-binding assays, the IR and avidities were determined for 1F5 and 1F5/SA using Ramos cells and Lym-1, Lym-1/SA, HD39, and HD39/SA using Raji cells. No statistically significant differences in avidity were seen between the Ab/SA conjugates and the corresponding unconjugated Abs (paired t test, two-tailed, P < .05).
IR indicates immunoreactivity.
All experiments were done in triplicate. Mean ± SD.