Second-order rate constants (k2) for the inhibition of aPC by PCI and ΔH-PCI in the presence of heparin (2.5 μg/mL) or phospholipids (50 μg/mL each)
| . | k2, M−1min−1, mean ± SEM . | Fold stimulation . |
|---|---|---|
| rPCI-wt | ||
| Buffer | 9.58 ± 0.90 × 104 | — |
| Heparin | 2.58 ± 0.05 × 107 | 269 |
| OxPAPE | 1.47 ± 0.31 × 107 | 153 |
| PAPE | 1.79 ± 0.63 × 105 | 1.9 |
| OxPAPS | 1.27 ± 0.11 × 107 | 133 |
| PAPS | 1.86 ± 0.06 × 107 | 194 |
| OxPAPC | 4.06 ± 1.13 × 104 | 0.4 |
| PAPC | 5.75 ± 1.35 × 104 | 0.6 |
| ΔH-PCI | ||
| Buffer | 1.27 ± 0.18 × 105 | — |
| Heparin | 1.31 ± 0.35 × 105 | 1.03 |
| PAPS | 1.32 ± 0.37 × 105 | 1.04 |
| . | k2, M−1min−1, mean ± SEM . | Fold stimulation . |
|---|---|---|
| rPCI-wt | ||
| Buffer | 9.58 ± 0.90 × 104 | — |
| Heparin | 2.58 ± 0.05 × 107 | 269 |
| OxPAPE | 1.47 ± 0.31 × 107 | 153 |
| PAPE | 1.79 ± 0.63 × 105 | 1.9 |
| OxPAPS | 1.27 ± 0.11 × 107 | 133 |
| PAPS | 1.86 ± 0.06 × 107 | 194 |
| OxPAPC | 4.06 ± 1.13 × 104 | 0.4 |
| PAPC | 5.75 ± 1.35 × 104 | 0.6 |
| ΔH-PCI | ||
| Buffer | 1.27 ± 0.18 × 105 | — |
| Heparin | 1.31 ± 0.35 × 105 | 1.03 |
| PAPS | 1.32 ± 0.37 × 105 | 1.04 |
— indicates not applicable.