RT-PCR primers used in this research
| Gene . | 5′ primer . | 3′ primer . | bp . |
|---|---|---|---|
| α-globin | TGGGGTAAGGTCGGCGCGCA | TGCACCGCAGGGGTGAACTC | 326 |
| β-globin | GGTGGTCTACCCTTGGACCC | GATACTTGTGGGCCAGGGCA | 341 |
| γ-globin | GGGAGATGCCATAAAGC | ATTGCCAAAACGGTCAC | 135 |
| DNMT-1 | ACCGCTTCTACTTCCTCGAGGCCTA | GTTGCAGTCCTCTGTGAACACTGTGG | 335 |
| DNMT-3a | CACACAGAAGCATATCCAGCAGTG | AGTGGACTGGGAAACCAAATACCC | 551 |
| DNMT-3b | AATGTGAATCCAGCCAGGAAAGGC | ACTGGATTACACTCCAGGAACCGT | 190 |
| G3PD | TCCCATCACCATCTTCCA | CATCACGCCACAGTTTCC | 380 |
| β-actin | GTGGGGCGCCCCAGGCACCA | CTCCTTAATGTCACGCACGATTTC | 540 |
| Gene . | 5′ primer . | 3′ primer . | bp . |
|---|---|---|---|
| α-globin | TGGGGTAAGGTCGGCGCGCA | TGCACCGCAGGGGTGAACTC | 326 |
| β-globin | GGTGGTCTACCCTTGGACCC | GATACTTGTGGGCCAGGGCA | 341 |
| γ-globin | GGGAGATGCCATAAAGC | ATTGCCAAAACGGTCAC | 135 |
| DNMT-1 | ACCGCTTCTACTTCCTCGAGGCCTA | GTTGCAGTCCTCTGTGAACACTGTGG | 335 |
| DNMT-3a | CACACAGAAGCATATCCAGCAGTG | AGTGGACTGGGAAACCAAATACCC | 551 |
| DNMT-3b | AATGTGAATCCAGCCAGGAAAGGC | ACTGGATTACACTCCAGGAACCGT | 190 |
| G3PD | TCCCATCACCATCTTCCA | CATCACGCCACAGTTTCC | 380 |
| β-actin | GTGGGGCGCCCCAGGCACCA | CTCCTTAATGTCACGCACGATTTC | 540 |