Platelet counts and human PF4 levels in inflammatory models in hPF4+ mice
| Injection . | Drop in platelet count, % of baseline . | PF4 plasma level, kU/mL . | PF4 level in lungs, U/mg protein . |
|---|---|---|---|
| PBS, 200 μL IV | 92 ± 11 | 3.65 ± 1.30 | 583 ± 271 |
| Thrombin, 80 U/kg IV | 72 ± 11* | 7.67 ± 1.00† | 1087 ± 99† |
| PBS, 200 μL IP | 100 ± 17 | 2.57 ± 0.90 | 656 ± 120 |
| LPS, 40 mg/kg IP | 75 ± 13* | 3.05 ± 0.77 | 991 ± 218† |
| Injection . | Drop in platelet count, % of baseline . | PF4 plasma level, kU/mL . | PF4 level in lungs, U/mg protein . |
|---|---|---|---|
| PBS, 200 μL IV | 92 ± 11 | 3.65 ± 1.30 | 583 ± 271 |
| Thrombin, 80 U/kg IV | 72 ± 11* | 7.67 ± 1.00† | 1087 ± 99† |
| PBS, 200 μL IP | 100 ± 17 | 2.57 ± 0.90 | 656 ± 120 |
| LPS, 40 mg/kg IP | 75 ± 13* | 3.05 ± 0.77 | 991 ± 218† |
Mice (n = 4-6 per arm) were injected with either phosphate-buffered saline (PBS), 80 U/kg thrombin or 40 mg/kg LPS. Compared are results of PBS solute only (200 μL/mouse) 10 minutes after thrombin (80 U/kg in 200 μL IV) and 2 hours after LPS (40 mg/kg in 200 μL IP). The means (± 1 SD) are shown. Statistical analysis between groups was performed using the Student t test (A-C). Note: Platelets release PF4 in vivo after either thrombin or LPS injection into mice.
P < .04 compared with simultaneous studies of PBS controls.
P < .02 compared with simultaneous studies of PBS controls.