Relative intensities of signaling proteins in FL compared with FH
| Signaling protein . | Relative signal intensity in FH (N = 22) . | Relative signal intensity in FL, all grades (N = 36) . | P* . | ||||
|---|---|---|---|---|---|---|---|
| Median . | SD . | Range . | Median . | SD . | Range . | ||
| Phospho Erk Thr202/Tyr204 | 4.11 | 3.09 | 0.96-10.46 | 15.83 | 23.19 | 2.28-100 | < .001 |
| Erk, total | 39.33 | 22.12 | 13.87-95.74 | 40.67 | 21.45 | 14.99-100 | .56 |
| Phospho-Mek 1/2 Ser217/221 | 14.79 | 10.77 | 4.68-44.44 | 25.37 | 23.75 | 7.5-100 | .003 |
| Mek 1/2, total | 39.29 | 13.68 | 21.61-69.88 | 41.99 | 23.86 | 18.24-100 | .58 |
| Phospho–Stat-6 Tyr641 | 3.44 | 3.34 | 0.58-12.06 | 6.24 | 18.28 | 1.25-100 | .012 |
| Stat-6, total | 17.54 | 19.38 | 4.95-100 | 19.53 | 18.94 | 3-95.11 | .88 |
| Signaling protein . | Relative signal intensity in FH (N = 22) . | Relative signal intensity in FL, all grades (N = 36) . | P* . | ||||
|---|---|---|---|---|---|---|---|
| Median . | SD . | Range . | Median . | SD . | Range . | ||
| Phospho Erk Thr202/Tyr204 | 4.11 | 3.09 | 0.96-10.46 | 15.83 | 23.19 | 2.28-100 | < .001 |
| Erk, total | 39.33 | 22.12 | 13.87-95.74 | 40.67 | 21.45 | 14.99-100 | .56 |
| Phospho-Mek 1/2 Ser217/221 | 14.79 | 10.77 | 4.68-44.44 | 25.37 | 23.75 | 7.5-100 | .003 |
| Mek 1/2, total | 39.29 | 13.68 | 21.61-69.88 | 41.99 | 23.86 | 18.24-100 | .58 |
| Phospho–Stat-6 Tyr641 | 3.44 | 3.34 | 0.58-12.06 | 6.24 | 18.28 | 1.25-100 | .012 |
| Stat-6, total | 17.54 | 19.38 | 4.95-100 | 19.53 | 18.94 | 3-95.11 | .88 |
RPMA of 36 samples of FL and 22 samples of FH, probed with antibodies for the proteins listed (“Methods”). Relative signal intensities representing protein content were generated using Microvigene imaging analysis softwear, normalized to total protein and scaled to the maximum value in the group. Standard deviations of the mean relative signal intensity and ranges are reported next to the median relative signal intensity for each protein.
The Mann-Whitney rank test was used to compare FL and FH with P values set at P = .01 for determination of significance, due to multiple comparisons.