Sequences of oligonucleotides used in the present study
| Name . | Sequence (5′ to 3′) . | Position in PROCR sequence (AF106202) . |
|---|---|---|
| Primers used for cDNA amplification of PROCR genotyping | ||
| EPCR 2347 Fr | CATTGCTGCCGATACTGCTGC | nt 2347 to 2367 |
| EPCR 4993 Fr | CCCAGACACCAACACCACG | nt 4993 to 5010 |
| EPCR 6970 Rv | AAGATGCCTACAGCCACACC | nt 6970 to 6951 |
| EPCR 7320 Rv | GTCTGTCTTTGGAGGATGGGCC | nt 7320 to 7299 |
| EPCR 7190 Rv | CAAGTACTTTGTCCACCTCTCC | nt 7190 to 7169 |
| EPCR 3960 Fr | GAGGCAGAGCAATCCTGACA | nt 3960 to 3980 |
| EPCR 4562 Rv | CATCTCCTCTTCCACTGCCC | nt 4526 to 4507 |
| EPCR 4069 Fr | GGGCCAGGTCTCTAGCAGCC | nt 4069 to 4088 |
| Primers used to clone the EPCR cDNA in the pCI-neo expression vector | ||
| First-round PCR | ||
| EPCR upstream | GGTCCGGAGCCTCAACTTCAG | nt 2315 to 2335 |
| EPCR downstream | ACTTTCCCTTGCCAGCCTCC | nt 7054 to 7035 |
| Nested PCR with oligonucleotides containing the cloning sites | ||
| EPCR Nhe IFr | GACTCACTATAGGGAGACCCAAGCTGgctagcTCAGGATGTTGACAACATTGCTGCCGATAC | |
| EPCR Not IRv | GGTGTTTCAGTTGGGGAGTCTCGAgcggccgcGAGCTGAAACTTTCCCTTGCCAGCCTCCATC | nt 7062 to 7032 |
| Primers used to clone the cDNA of EPCR 1–209 (rEPCRsol) in the pCI-neo expression vector | ||
| pCI-neo upstream 2 | CGAGACAGAGAAGACTCTTGCGTTTC | [in plasmid pCI-neo sequence] |
| EPCRsol1–209NotI | CACAAGGAAAAGGTATTAgcggccgcTTAAGTGTAGGAGCGGCTTGTTTGGCTC | nt 6908 to 6884 |
| Primers used to clone the cDNA corresponding to EPCR isoform (rEPCRisoform) in the pCI-neo expression vector | ||
| 5′ part of the cDNA | ||
| pCI-neo upstream 2 | CGAGACAGAGAAGACTCTTGCGTTTC | [in plasmid pCI-neo sequence] |
| EPCR 6568Rv | CCCGCAGTTCATACCGAGTG | nt 6568 to 6549 |
| 3′ part of the cDNA | ||
| EPCR 4993Fr | CCCAGACACCAACACCACG | nt 4993 to 5012 |
| EPCR 7480 Rv Not I | CACAAGGAAAGGTATTAgcggccgcGGGATTTTACATTTCCACCACTTCTTCGTG | nt 7480 to 7451 |
| Name . | Sequence (5′ to 3′) . | Position in PROCR sequence (AF106202) . |
|---|---|---|
| Primers used for cDNA amplification of PROCR genotyping | ||
| EPCR 2347 Fr | CATTGCTGCCGATACTGCTGC | nt 2347 to 2367 |
| EPCR 4993 Fr | CCCAGACACCAACACCACG | nt 4993 to 5010 |
| EPCR 6970 Rv | AAGATGCCTACAGCCACACC | nt 6970 to 6951 |
| EPCR 7320 Rv | GTCTGTCTTTGGAGGATGGGCC | nt 7320 to 7299 |
| EPCR 7190 Rv | CAAGTACTTTGTCCACCTCTCC | nt 7190 to 7169 |
| EPCR 3960 Fr | GAGGCAGAGCAATCCTGACA | nt 3960 to 3980 |
| EPCR 4562 Rv | CATCTCCTCTTCCACTGCCC | nt 4526 to 4507 |
| EPCR 4069 Fr | GGGCCAGGTCTCTAGCAGCC | nt 4069 to 4088 |
| Primers used to clone the EPCR cDNA in the pCI-neo expression vector | ||
| First-round PCR | ||
| EPCR upstream | GGTCCGGAGCCTCAACTTCAG | nt 2315 to 2335 |
| EPCR downstream | ACTTTCCCTTGCCAGCCTCC | nt 7054 to 7035 |
| Nested PCR with oligonucleotides containing the cloning sites | ||
| EPCR Nhe IFr | GACTCACTATAGGGAGACCCAAGCTGgctagcTCAGGATGTTGACAACATTGCTGCCGATAC | |
| EPCR Not IRv | GGTGTTTCAGTTGGGGAGTCTCGAgcggccgcGAGCTGAAACTTTCCCTTGCCAGCCTCCATC | nt 7062 to 7032 |
| Primers used to clone the cDNA of EPCR 1–209 (rEPCRsol) in the pCI-neo expression vector | ||
| pCI-neo upstream 2 | CGAGACAGAGAAGACTCTTGCGTTTC | [in plasmid pCI-neo sequence] |
| EPCRsol1–209NotI | CACAAGGAAAAGGTATTAgcggccgcTTAAGTGTAGGAGCGGCTTGTTTGGCTC | nt 6908 to 6884 |
| Primers used to clone the cDNA corresponding to EPCR isoform (rEPCRisoform) in the pCI-neo expression vector | ||
| 5′ part of the cDNA | ||
| pCI-neo upstream 2 | CGAGACAGAGAAGACTCTTGCGTTTC | [in plasmid pCI-neo sequence] |
| EPCR 6568Rv | CCCGCAGTTCATACCGAGTG | nt 6568 to 6549 |
| 3′ part of the cDNA | ||
| EPCR 4993Fr | CCCAGACACCAACACCACG | nt 4993 to 5012 |
| EPCR 7480 Rv Not I | CACAAGGAAAGGTATTAgcggccgcGGGATTTTACATTTCCACCACTTCTTCGTG | nt 7480 to 7451 |
Restriction site sequences are shown in lowercase letters. The sequences complementary to the EPCR cDNA are in bold letters.