Identification of high and low responders among CMV-seropositive donors
| Donor . | CMV status . | Fkn levels, ng/mL . | IFN-γ response by ELISPOT per 100 000 PBMC . |
|---|---|---|---|
| 1 High | Positive | 252 | 530 |
| 2 High | Positive | 249 | 400 |
| 3 High | Positive | 170 | 140 |
| 4 High | Positive | 199 | 500 |
| 5 High | Positive | 180 | 230 |
| 6 Low | Positive | 22 | 20 |
| 7 Low | Positive | 20 | 60 |
| 8 Low | Positive | 41.5 | 50 |
| 9 | Negative | < 3 | Below detection |
| Donor . | CMV status . | Fkn levels, ng/mL . | IFN-γ response by ELISPOT per 100 000 PBMC . |
|---|---|---|---|
| 1 High | Positive | 252 | 530 |
| 2 High | Positive | 249 | 400 |
| 3 High | Positive | 170 | 140 |
| 4 High | Positive | 199 | 500 |
| 5 High | Positive | 180 | 230 |
| 6 Low | Positive | 22 | 20 |
| 7 Low | Positive | 20 | 60 |
| 8 Low | Positive | 41.5 | 50 |
| 9 | Negative | < 3 | Below detection |
A group of CMV-seropositive donors was compared for induced fractalkine levels, measured by ELISA as ng per mL in coculture supernatants. The CMV-seronegative donor has a fractalkine level of less than 3 ng/mL, which is consistent with several other CMV-seronegative donors screened.20 The ELISPOT assay results for each donor are presented as number of IFN-γ−positive cells per 100 000 PBMC. The ELISPOT ranges for frequencies of CMV-specific responding T cells were also consistent with a flow cytometry analysis done on the same samples, where intracellular IFN-γ + CD4+ T cells activated in response to CMV antigen were detected.