Table 2

Effect of stirring on recombinant human latent TGF-β1 (rhLTGF-β1)

ELISAExperiment
Mean (SD)% Active (SD)
12345
Active TGF-β1, ng/mL        
    Unstirred 0.55 0.38 0.35 0.41 0.39 0.41 (0.08) 1.0 (0.08) 
    Stirred 0.68 0.41 0.45 0.44 0.44 0.48 (0.11)* 1.3 (0.16) 
Total TGF-β1, ng/mL        
    Unstirred 58 37 37 40 37 41 (9)  
    Stirred 58 30 34 33 31 37 (12)*  
ELISAExperiment
Mean (SD)% Active (SD)
12345
Active TGF-β1, ng/mL        
    Unstirred 0.55 0.38 0.35 0.41 0.39 0.41 (0.08) 1.0 (0.08) 
    Stirred 0.68 0.41 0.45 0.44 0.44 0.48 (0.11)* 1.3 (0.16) 
Total TGF-β1, ng/mL        
    Unstirred 58 37 37 40 37 41 (9)  
    Stirred 58 30 34 33 31 37 (12)*  
*

P = .02 versus unstirred.

P = .002 versus unstirred.

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