Characteristics of the T- ALL cell lines included in this study
| Characteristics . | Cell line . | |||
|---|---|---|---|---|
| CEM-C7 . | CEM-C1 . | Jurkat . | MOLT-4 . | |
| IC50 DEX, μM | 0.8 | >10 | > 10 | 10 |
| IC50 PRED, μM | 1.8 | >100 | > 100 | > 100 |
| ATO, μM | 2.0 | 1.8 | 1.2 | 0.76 |
| GR status | L753F het | L753F het | R477H het | ? |
| p53 status | het mut | het mut | het mut | no mutation |
| Nutlin assay | no effect | no effect | no effect | inhibition |
| Characteristics . | Cell line . | |||
|---|---|---|---|---|
| CEM-C7 . | CEM-C1 . | Jurkat . | MOLT-4 . | |
| IC50 DEX, μM | 0.8 | >10 | > 10 | 10 |
| IC50 PRED, μM | 1.8 | >100 | > 100 | > 100 |
| ATO, μM | 2.0 | 1.8 | 1.2 | 0.76 |
| GR status | L753F het | L753F het | R477H het | ? |
| p53 status | het mut | het mut | het mut | no mutation |
| Nutlin assay | no effect | no effect | no effect | inhibition |
IC50s were calculated using Graphpad Prism software with triplicate values from MTT assays. The GR mutation R477H was also found in families with generalized resistance to GC, mutation L753F was found in the patient samples from which the CEM line was derived, and probably is not relevant for GC-resistance in CEM-C1. p53 mutations as reported in the literature. We determined the dose response curves for nutlin-3 with the MTT assay.
het indicates heterozygous; mut, mutation; DEX, dexamethasone; and PRED, prednisone.
? indicates mutation status unknown.