Combined data from Figures 4 and 5 for limiting-dilution analysis of human cord blood CD133+ cells transplanted into NOD/SCID mice before and after culture at normal or low oxygen levels
| Input equivalent cell no. . | 0.1% engraftment as cutoff . | 1% engraftment as cutoff . | ||
|---|---|---|---|---|
| Positive mice vs total mice . | 1/CRU . | Positive mice vs total mice . | 1/CRU . | |
| Uncultured CD133+ cells | 10256 | 43992 | ||
| 5000 | 6/9 | — | 1/9 | — |
| 8000 | 2/7 | — | 1/7 | — |
| 10000 | 7/8 | — | 1/8 | — |
| 15000 | 4/8 | — | 4/8 | — |
| 20000 | 7/8 | — | 2/8 | — |
| Cultured CD133+ cells at normal O2 | <1667 | 3235 | ||
| 1667 | 7/7 | — | 3/7 | — |
| 5000 | 10/10 | — | 7/10 | — |
| 10000 | 10/10 | — | 10/10 | — |
| Cultured CD133+ cells at 5% O2 | <1667 | 5034 | ||
| 1667 | 6/6 | — | 2/6 | — |
| 5000 | 10/10 | — | 4/10 | — |
| 8000 | 8/8 | — | 8/8 | — |
| Input equivalent cell no. . | 0.1% engraftment as cutoff . | 1% engraftment as cutoff . | ||
|---|---|---|---|---|
| Positive mice vs total mice . | 1/CRU . | Positive mice vs total mice . | 1/CRU . | |
| Uncultured CD133+ cells | 10256 | 43992 | ||
| 5000 | 6/9 | — | 1/9 | — |
| 8000 | 2/7 | — | 1/7 | — |
| 10000 | 7/8 | — | 1/8 | — |
| 15000 | 4/8 | — | 4/8 | — |
| 20000 | 7/8 | — | 2/8 | — |
| Cultured CD133+ cells at normal O2 | <1667 | 3235 | ||
| 1667 | 7/7 | — | 3/7 | — |
| 5000 | 10/10 | — | 7/10 | — |
| 10000 | 10/10 | — | 10/10 | — |
| Cultured CD133+ cells at 5% O2 | <1667 | 5034 | ||
| 1667 | 6/6 | — | 2/6 | — |
| 5000 | 10/10 | — | 4/10 | — |
| 8000 | 8/8 | — | 8/8 | — |
— indicates not applicable.