Differentiation of T1 and T2 B lymphocytes
| Differentiation, stimulus . | 24 hours . | 48 hours . |
|---|---|---|
| T1 to T2 | ||
| Medium | ND | 9.90 ± 4.67 |
| Anti-CD38 | ND | 12.41 ± 5.54 |
| T2 to M | ||
| Medium | 10.66 ± 1.15 | 13.66 ± 4.04 |
| Rat-IgG2a | 8.33 ± 2.0 | 9.33 ± 2.04 |
| Anti-CD38 | 60.33 ± 2.52 | 77.66 ± 3.33* |
| Differentiation, stimulus . | 24 hours . | 48 hours . |
|---|---|---|
| T1 to T2 | ||
| Medium | ND | 9.90 ± 4.67 |
| Anti-CD38 | ND | 12.41 ± 5.54 |
| T2 to M | ||
| Medium | 10.66 ± 1.15 | 13.66 ± 4.04 |
| Rat-IgG2a | 8.33 ± 2.0 | 9.33 ± 2.04 |
| Anti-CD38 | 60.33 ± 2.52 | 77.66 ± 3.33* |
Purified T1 or T2 B subsets from wild-type mice were incubated 24 or 48 hours as indicated. The differentiation is expressed by the percentage of B cell subsets in T2 or M regions (see Figures 3A,S3B). The results are expressed as means plus or minus SD from 3 independent experiments.
ND indicates not determined.
P <.01, comparing the differentiation induced by presence or absence of anti-CD38 (Student t test).