Altered differentiation of T2 B cells in mice with signaling defects
| Stimulus . | Mice . | ||||
|---|---|---|---|---|---|
| WT . | CD38−/− . | XID . | Lyn−/− . | Fyn−/− . | |
| Rat-IgG | 13.61 ± 4.01 | 13.01 ± 1.10 | 10.33 ± 2.08 | 8.03 ± 2.10 | 13.33 ± 4.04 |
| Anti-CD38 | 74.83 ± 3.78 | 19.11 ± 2.01* | 13.33 ± 1.52* | 18.10 ± 1.0* | 20.66 ± 4.6* |
| Stimulus . | Mice . | ||||
|---|---|---|---|---|---|
| WT . | CD38−/− . | XID . | Lyn−/− . | Fyn−/− . | |
| Rat-IgG | 13.61 ± 4.01 | 13.01 ± 1.10 | 10.33 ± 2.08 | 8.03 ± 2.10 | 13.33 ± 4.04 |
| Anti-CD38 | 74.83 ± 3.78 | 19.11 ± 2.01* | 13.33 ± 1.52* | 18.10 ± 1.0* | 20.66 ± 4.6* |
Purified T2 B cells from wild-type mice or mice deficient in the expression of CD38, Btk (XID), Lyn, or Fyn were stimulated 48 hours with anti-CD38. The differentiation of T2 B cells induced by anti-CD38 is expressed as the percentage of cells in the region of mature B lymphocytes (Figure 6A). The results are expressed as mean and standard deviation from 3 independent experiments
P <.01, comparing anti-CD38 induced differentiation among the different mice studied (Student t test).