Inhibition of T2 B lymphocyte differentiation by drugs interfering with signaling
| Stimulus . | Inhibitor . | ||||
|---|---|---|---|---|---|
| DMSO . | PD98059 . | U-73122 . | U-73343 . | LY294002 . | |
| Rat-IgG | 10.09 ± 1.73 | 8.66 ± 3.03 | 10.66 ± 1.01 | 9.33 ± 1.52 | 10.33 ± 1.05 |
| Anti-CD38 | 73.31 ± 5.0 | 23.33 ± 4.55* | 66.43 ± 3.78 | 68.66 ± 3.5 | 16.66 ± 2.1* |
| Stimulus . | Inhibitor . | ||||
|---|---|---|---|---|---|
| DMSO . | PD98059 . | U-73122 . | U-73343 . | LY294002 . | |
| Rat-IgG | 10.09 ± 1.73 | 8.66 ± 3.03 | 10.66 ± 1.01 | 9.33 ± 1.52 | 10.33 ± 1.05 |
| Anti-CD38 | 73.31 ± 5.0 | 23.33 ± 4.55* | 66.43 ± 3.78 | 68.66 ± 3.5 | 16.66 ± 2.1* |
T2 B lymphocytes from wild-type mice were preincubated 30 minutes with DMSO or one of the indicated inhibitors and then stimulated 48 hours with anti-CD38. The differentiation induced by anti-CD38 is expressed as the percentage of cells in the region of mature B lymphocytes (Figure 6B). The results are expressed as mean ± SD from 3 independent experiments.
P < .01, comparing anti-CD38 induced differentiation in the presence of different drugs or DMSO (Student t test).