Hematology of humanized HbA control, β thalassemia minor, and β thalassemia major mice before and after transfusion
| Human Hb mice . | n . | RBC, 106/μL . | Hb, g/dL . | PCV, % . | Retic, % . | RDW, % . | Spleen, % of BW . |
|---|---|---|---|---|---|---|---|
| HbA control | 9 | 10.9 (± 0.3) | 13.6 (± 0.1) | 44.8 (± 0.4) | 2.8 (± 0.2) | 19.6 (± 0.2) | 0.35 (± 0.02) |
| β thalassemia minor | 10 | 11.8 (± 0.2)* | 12.7 (± 0.3)* | 42.0 (± 0.7)* | 4.1 (± 0.3)* | 20.2 (± 0.3)n.s. | 0.53 (± 0.04)* |
| β thalassemia major untransfused CA | 9 | 2.5 (± 0.3)** | 3.0 (± 0.4)** | 15.0 (± 1.9)** | 72.1 (± 2.2)** | 38.5 (± 2.4)** | 5.1 (± 1.5)* |
| β thalassemia major hypertransfused CA | 5 | 9.8 (± 0.5)n.s. | 15.1 (± 0.6)* | 42.2 (± 1.8)n.s. | ND | 16.0 (± 0.2)** | 0.79 (± 0.07)** |
| β thalassemia major hypotransfused CA | 5 | 5.9 (± 0.1)** | 7.4 (± 0.4)** | 25.8 (± 0.7)** | ND | 18.4 (± 0.5)* | 4.1 (± 0.4)** |
| Human Hb mice . | n . | RBC, 106/μL . | Hb, g/dL . | PCV, % . | Retic, % . | RDW, % . | Spleen, % of BW . |
|---|---|---|---|---|---|---|---|
| HbA control | 9 | 10.9 (± 0.3) | 13.6 (± 0.1) | 44.8 (± 0.4) | 2.8 (± 0.2) | 19.6 (± 0.2) | 0.35 (± 0.02) |
| β thalassemia minor | 10 | 11.8 (± 0.2)* | 12.7 (± 0.3)* | 42.0 (± 0.7)* | 4.1 (± 0.3)* | 20.2 (± 0.3)n.s. | 0.53 (± 0.04)* |
| β thalassemia major untransfused CA | 9 | 2.5 (± 0.3)** | 3.0 (± 0.4)** | 15.0 (± 1.9)** | 72.1 (± 2.2)** | 38.5 (± 2.4)** | 5.1 (± 1.5)* |
| β thalassemia major hypertransfused CA | 5 | 9.8 (± 0.5)n.s. | 15.1 (± 0.6)* | 42.2 (± 1.8)n.s. | ND | 16.0 (± 0.2)** | 0.79 (± 0.07)** |
| β thalassemia major hypotransfused CA | 5 | 5.9 (± 0.1)** | 7.4 (± 0.4)** | 25.8 (± 0.7)** | ND | 18.4 (± 0.5)* | 4.1 (± 0.4)** |
Genotypes of human Hb mice are as follows: HbA (α2α1/α2α1 γβA/γβA), β thalassemia minor (α2α1/α2α1 γHPFHδβ0/γβA), and β thalassemia major (α2α1/α2α1 γHPFHδβ0/γHPFHδβ0). Mice are analyzed 8 weeks after birth, except the untransfused CA mice, which are analyzed at the time of their euthanasia (age of 19 ± 6 days). Values represent mean (± SEM). Statistical significances are determined for the β thalassemic major and minor mice compared with the HbA control mice. P values were calculated by 2-tailed unpaired Student t test.
RBC indicates red blood cell; Hb, hemoglobin; PCV, packed cell volume; Retic, reticulocyte; RDW, red cell distribution width; BW, body weight; ND, not determined; and n.s., not significant.
P < .05.
P < .0001.