Total FL cell suspensions from embryonic day 12.5 Wt or Wnt3a−/− embryos (Ly5.2) were prepared and the indicated numbers of cells were intravenously injected into sublethally irradiated Ly5.1 recipient mice
| E12.5 FL cells/mouse . | Repopulated mice . | |
|---|---|---|
| Wt . | Wnt3a−/− . | |
| 0.25 × 106 | 2/6* (33%)† | 0/6 (0%) |
| 1 × 106 | 3/5 (60%) | 0/3 (0%) |
| 4 × 106 | 4/4 (100%) | 3/3 (100%) |
| E12.5 FL cells/mouse . | Repopulated mice . | |
|---|---|---|
| Wt . | Wnt3a−/− . | |
| 0.25 × 106 | 2/6* (33%)† | 0/6 (0%) |
| 1 × 106 | 3/5 (60%) | 0/3 (0%) |
| 4 × 106 | 4/4 (100%) | 3/3 (100%) |