Free λLC production over time
| Length of incubation, d . | ALMC-1, ng/mL free λLC (total μg free λLC) . | ALMC-2, ng/mL free λLC (total μg free λLC) . |
|---|---|---|
| 1 | 0.34 (13.7) | 0.26 (10.6) |
| 3 | 1.47 (58.7) | 1.65 (66) |
| 5 | 4.5 (180) | 5.2 (208) |
| 7 | 9.1 (364) | 8.7 (349) |
| Length of incubation, d . | ALMC-1, ng/mL free λLC (total μg free λLC) . | ALMC-2, ng/mL free λLC (total μg free λLC) . |
|---|---|---|
| 1 | 0.34 (13.7) | 0.26 (10.6) |
| 3 | 1.47 (58.7) | 1.65 (66) |
| 5 | 4.5 (180) | 5.2 (208) |
| 7 | 9.1 (364) | 8.7 (349) |
ALMC-1 and ALMC-2 cells were seeded at an initial density of 200 000 cells/mL in 40 mL of complete media supplemented with IL-6. Cell-free supernatants were harvested, and free λLC levels per mL and total production were determined.