Quantification of VAMP isoforms in human and mouse platelets
| . | Human, n=3 . | WT mouse, n=3 . | VAMP-8−/− mouse, n=3 . | |||
|---|---|---|---|---|---|---|
| ng/5×107* . | Molecules/plt† . | ng/5×107 . | Molecules/plt . | ng/5×107 . | Molecules/plt . | |
| VAMP-2 | < 1 | < 806 | 9.46 ± 0.11 | 7629 ± 89 | 9.06 ± 0.51 | 7306 ± 411 |
| VAMP-3 | 4.59 ± 0.66 | 4588 ± 658 | 2.20 ± 0.12 | 2197 ± 120 | 1.92 ± 0.19 | 1920 ± 190 |
| VAMP-7 | 7.84 ± 1.45 | 3766 ± 696 | 5.65 ± 0.49 | 2712 ± 230 | 5.56 ± 1.30 | 2669 ± 624 |
| VAMP-8 | 6.6 ± 0.37 | 6590 ± 370 | 8.36 ± 0.11 | 8360 ± 110 | ND | ND |
| . | Human, n=3 . | WT mouse, n=3 . | VAMP-8−/− mouse, n=3 . | |||
|---|---|---|---|---|---|---|
| ng/5×107* . | Molecules/plt† . | ng/5×107 . | Molecules/plt . | ng/5×107 . | Molecules/plt . | |
| VAMP-2 | < 1 | < 806 | 9.46 ± 0.11 | 7629 ± 89 | 9.06 ± 0.51 | 7306 ± 411 |
| VAMP-3 | 4.59 ± 0.66 | 4588 ± 658 | 2.20 ± 0.12 | 2197 ± 120 | 1.92 ± 0.19 | 1920 ± 190 |
| VAMP-7 | 7.84 ± 1.45 | 3766 ± 696 | 5.65 ± 0.49 | 2712 ± 230 | 5.56 ± 1.30 | 2669 ± 624 |
| VAMP-8 | 6.6 ± 0.37 | 6590 ± 370 | 8.36 ± 0.11 | 8360 ± 110 | ND | ND |
ND indicates not detected.
Mass of the VAMP in nanograms per 5 × 107 platelets was determined by quantitative Western blotting using recombinant VAMPs to generate a standard curve.
Number of molecules per platelet was calculated using the following molecular weights for the indicated VAMP: VAMP-2, 13.5 kDa; VAMP-3, 12 kDa; VAMP-7, 25 kDa; VAMP-8, 12 kDa.