Progenitor frequency analysis of human hematopoietic stem cell subsets
| HSC subset* . | Progenitor frequency−1 (95% confidence limits)† . |
|---|---|
| CD34+CD38− | 4.76 (3.66-6.21) |
| CD34+CD38lo | 3.85 (2.94-5.06) |
| CD34+CD38+/hi | 19.30 (14.77-25.22) |
| HSC subset* . | Progenitor frequency−1 (95% confidence limits)† . |
|---|---|
| CD34+CD38− | 4.76 (3.66-6.21) |
| CD34+CD38lo | 3.85 (2.94-5.06) |
| CD34+CD38+/hi | 19.30 (14.77-25.22) |
CD34+CD38−, CD34+CD38lo, and CD34+CD38+/hi HSCs were placed at limiting numbers in wells of a 96-well/plate containing OP9-DL1 cells, and cultured for 11 days before harvesting for flow cytometric analysis.
Individual wells were scored for the presence of T cells based on CD45+CD7++ staining. Statistical analysis was performed via the method of maximum likelihood applied to the Poisson model.13