Progenitor frequency analysis of progenitor T-cell subsets
| ProT subset* . | Culture system† . | Progenitor frequency−1 (95% confidence limits)‡ . |
|---|---|---|
| ProT1, CD34+CD7+CD5− | FTOC | 1384.72 (979-1959) |
| ProT2, CD34+CD7+CD5+ | FTOC | 411.74 (256-663) |
| ProT1, CD34+CD7+CD5− | OP9-DL1 | 2.52 (1.75-3.63) |
| ProT2, CD34+CD7+CD5+ | OP9-DL1 | 1.95 (1.35-2.80) |
| ProT subset* . | Culture system† . | Progenitor frequency−1 (95% confidence limits)‡ . |
|---|---|---|
| ProT1, CD34+CD7+CD5− | FTOC | 1384.72 (979-1959) |
| ProT2, CD34+CD7+CD5+ | FTOC | 411.74 (256-663) |
| ProT1, CD34+CD7+CD5− | OP9-DL1 | 2.52 (1.75-3.63) |
| ProT2, CD34+CD7+CD5+ | OP9-DL1 | 1.95 (1.35-2.80) |
CD34+ CD38−/lo UCB-derived cells were cultured on OP9-DL1 cells for 12 to 14 days and proT1/proT2 cells, with the indicated phenotypes, were obtained by flow cytometric cell sorting.
ProT subsets were placed in limiting numbers in FTOC or in wells of a 96-well/plate containing OP9-DL1 cells and cultured for 7 days before harvesting for flow cytometric analysis.
Individual lobes or wells were scored for the presence of T cells based on CD45+CD7++ or CD7++CD1a−/+ staining, respectively. Statistical analysis was performed via the method of maximum likelihood applied to the Poisson model.13