Table 1 Time-course studies of γ-globin... | American Society of Hematology

Table 1

Time-course studies of γ-globin gene activation by drug treatments in K562 cells

Compound	Drugs	24 h, g/GAPD mRNA			48 h, GAPD mRNA			72 h, GAPD mRNA
Compound	Drugs	Avg ± SD	P	Fold increase	Avg ± SD	P	Fold increase	Avg ± SD	P	Fold increase
Untreated	None	1.17 ± 0.62	–	1.00	0.80 ± 0.20	–	1.00	6.36 ± 1.81	–	1.00
Hemin	50μM	3.15 ± 1.13	.016	2.69	4.68 ± 0.28	0.0001	5.89	17.23 ± 3.26	.007	2.71
Butyrate	2mM	1.45 ± 0.37	.441	1.24	2.77 ± 0.76	0.0008	3.46	17.63 ± 2.16	.002	2.77
Trichostatin A	0.5μM	1.70 ± 0.16	.200	1.46	2.21 ± 0.59	0.0023	2.76	11.64 ± 1.51	.018	1.83
SAHA	5μM	1.53 ± 0.18	.368	1.31	1.37 ± 0.23	0.0052	1.71	12.34 ± 0.83	.007	1.94

Compound	Drugs	24 h, g/GAPD mRNA			48 h, GAPD mRNA			72 h, GAPD mRNA
Compound	Drugs	Avg ± SD	P	Fold increase	Avg ± SD	P	Fold increase	Avg ± SD	P	Fold increase
Untreated	None	1.17 ± 0.62	–	1.00	0.80 ± 0.20	–	1.00	6.36 ± 1.81	–	1.00
Hemin	50μM	3.15 ± 1.13	.016	2.69	4.68 ± 0.28	0.0001	5.89	17.23 ± 3.26	.007	2.71
Butyrate	2mM	1.45 ± 0.37	.441	1.24	2.77 ± 0.76	0.0008	3.46	17.63 ± 2.16	.002	2.77
Trichostatin A	0.5μM	1.70 ± 0.16	.200	1.46	2.21 ± 0.59	0.0023	2.76	11.64 ± 1.51	.018	1.83
SAHA	5μM	1.53 ± 0.18	.368	1.31	1.37 ± 0.23	0.0052	1.71	12.34 ± 0.83	.007	1.94

Data for each time point, shown as mean ± SD, are represented by 3 to 5 independent drug inductions.