Table 1 Kinetic values ( k cat and K m )... | American Society of Hematology

Table 1

Kinetic values (k_cat and K_m) and the catalytic efficiency (k_cat/K_m) derived for proteolysis of VWF115 and VWF115(D1614A) by both ADAMTS13 and ADAMTS13(R349A)

	VWF 115			VWF115(D1614A)
	K_m, μM	k_cat, s⁻¹	k_cat/K_m, ×10⁵ M⁻¹s⁻¹	K_m, _μM	k_cat, s⁻¹	k_cat/K_m, ×10⁵ M⁻¹s⁻¹
ADAMTS13	1.88 ± 0.73	0.59 ± 0.13	3.24 ± 0.57	7.99 ± 2.38	0.37 ± 0.04	0.50 ± 0.17
ADAMTS13(R349A)	6.20 ± 0.56	0.09 ± 0.01	0.15 ± 0.01	8.75 ± 2.34	0.47 ± 0.04	0.57 ± 0.13

	VWF 115			VWF115(D1614A)
	K_m, μM	k_cat, s⁻¹	k_cat/K_m, ×10⁵ M⁻¹s⁻¹	K_m, _μM	k_cat, s⁻¹	k_cat/K_m, ×10⁵ M⁻¹s⁻¹
ADAMTS13	1.88 ± 0.73	0.59 ± 0.13	3.24 ± 0.57	7.99 ± 2.38	0.37 ± 0.04	0.50 ± 0.17
ADAMTS13(R349A)	6.20 ± 0.56	0.09 ± 0.01	0.15 ± 0.01	8.75 ± 2.34	0.47 ± 0.04	0.57 ± 0.13

All data are mean of 3 separate experiments ± SD.