Table 1 Nested set of glutathione... | American Society of Hematology

Table 1

Nested set of glutathione S-transferase–HPA-1a–derived peptides

Peptide	Fragment	Sequence	Length	Observed [M + 2H]²⁺ ions	Calculated* mass [M⁺]	Theoretical mass [M⁺]
1	21-37	PMCAWCSDEALPLGSPR	17	916.2	1831.8	1830.5
2	22-37	MCAWCSDEALPLGSPR	16	934.1	1864.7	1866.2
3	24-37	AWCSDEALPLGSPR	14	751.4	1500.7	1500.8
4	25-37	WCSDEALPLGSPR	13	715.9	1429.6	1429.7

Peptide	Fragment	Sequence	Length	Observed [M + 2H]²⁺ ions	Calculated* mass [M⁺]	Theoretical mass [M⁺]
1	21-37	PMCAWCSDEALPLGSPR	17	916.2	1831.8	1830.5
2	22-37	MCAWCSDEALPLGSPR	16	934.1	1864.7	1866.2
3	24-37	AWCSDEALPLGSPR	14	751.4	1500.7	1500.8
4	25-37	WCSDEALPLGSPR	13	715.9	1429.6	1429.7

LC-MS/MS data were submitted to a Mascot search to identify proteins in the original sample. Four peptides derived from the recombinant HPA-1a antigen were identified in different fractions. All are presumed recovered from the HLA-DRB3*0101 molecules present on the HHKB cells.

Calculated mass = ([M + 2H]²⁺ × 2) − 2.

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