Relation between cell numbers and engraftment levels of cultured T-ALL cells
| T-ALL sampleMouse . | −GSI . | +GSI . | ||
|---|---|---|---|---|
| Injected cells . | CD45+ cells, % . | Injected cells . | CD45+ cells, % . | |
| M22 | ||||
| 1 | 197 000 | 84 | 25 000 | 1 |
| 2 | 225 000 | 0.5 | 54 000 | 0 |
| 3 | 230 000 | 60 | 11 000 | 0 |
| 4 | 230 000 | 50 | 52 000 | 50 |
| 5 | 83 000 | 0.5 | 35 000 | 0 |
| M30 | ||||
| 1 | 81 000 | 0.3 | 29 500 | 0 |
| 2 | 61 000 | 0.8 | 31 500 | 0 |
| 3 | 56 000 | 74 | 35 000 | 0 |
| 4 | 74 000 | 90 | 21 000 | 0 |
| T-ALL sampleMouse . | −GSI . | +GSI . | ||
|---|---|---|---|---|
| Injected cells . | CD45+ cells, % . | Injected cells . | CD45+ cells, % . | |
| M22 | ||||
| 1 | 197 000 | 84 | 25 000 | 1 |
| 2 | 225 000 | 0.5 | 54 000 | 0 |
| 3 | 230 000 | 60 | 11 000 | 0 |
| 4 | 230 000 | 50 | 52 000 | 50 |
| 5 | 83 000 | 0.5 | 35 000 | 0 |
| M30 | ||||
| 1 | 81 000 | 0.3 | 29 500 | 0 |
| 2 | 61 000 | 0.8 | 31 500 | 0 |
| 3 | 56 000 | 74 | 35 000 | 0 |
| 4 | 74 000 | 90 | 21 000 | 0 |
T-ALL blasts (105 cells/well) were cultured during 4 weeks in contact with MS5-DL1 (M22) and MS5-GFP (M30) cells. One week before the termination of culture, 10 μM/mL GSI was added in medium refreshments. At the end of the culture period, every well was recovered, cells were counted, and each sample was individually transplanted into a single NOD-Scid mouse. Human cell engraftment levels (measured by FACS, using mouse antihuman CD45 antibodies) in the injected bone marrow were analyzed 2 months after transplantation.