C/EBPα directly affects changes in myeloid and lymphoid gene expression in Cebpa/EYFP+ LSK cells, whereas the down-regulation of stem cell-specific genes in this cell population is only in part dependent on C/EBPα
| . | . | CebpaCre/+ LSK cells (EYFP+ vs EYFP−) . | Cebpanull LSK cells (EYFP+ vs EYFP−) . |
|---|---|---|---|
| Up-regulation | |||
| S100a8 | S-100 calcium-binding protein A8 | 55.8 | 0.76 |
| Ear2 | Eosinophil-associated ribonuclease A | 32.4 | 3.2 |
| Ly6c1 | Ly6c antigen, locus C1 | 21.6 | 0.83 |
| Ctsg | Cathepsin G | 14.5 | 1.8 |
| Ccr2 | Chemokine receptor 2 | 8.6 | 1.3 |
| Fcgr3 | Fc receptor, IgG, high-affinity III | 8.0 | 2.0 |
| Irf8 | Interferon regulatory factor 8 | 6.6 | 0.72 |
| Vcam1 | Vascular cell adhesion molecule 1 | 5.8 | 1.2 |
| Mpo | Myeloperoxidase | 5.2 | 1.0 |
| Csf1r | M-CSF receptor | 5.1 | 1.6 |
| Id2 | Inhibitor of DNA binding 2 | 4.4 | 1.4 |
| Csfr2b | GM-CSF receptor, subunit b | 2.5 | 0.45 |
| Lyz1 | Lysozyme 1 | 1.9 | 0.53 |
| Down-regulation | |||
| Evi1 | Ectropic viral integration site 1 | 0.18 | 0.03 |
| Ndn | Necdin | 0.21 | 0.4 |
| Rag1 | Recombination activating gene 1 | 0.23 | 1.5 |
| Ebf1 | Early B-cell factor 1 | 0.28 | 2.4 |
| Procr | Protein C receptor, endothelial | 0.29 | 0.23 |
| Mll5 | Mixed-lineage leukemia 5 | 0.35 | 1.76 |
| Vpreb1 | Pre-B lymphocyte gene 1 | 0.36 | 0.94 |
| Hoxa9 | Homeobox A9 | 0.38 | 0.65 |
| Pik3cd | PI3-kinase catalytic delta polypeptide | 0.39 | 1.27 |
| Foxo3 | Forkhead box O3 | 0.40 | 0.84 |
| Hoxa5 | Homeobox A5 | 0.41 | 0.83 |
| Tcf4 | Transcription factor 4 | 0.43 | 1.68 |
| Mycn | v-myc related, neuroblastoma derived | 0.48 | 0.34 |
| Ccr9 | Chemokine receptor 9 | 0.50 | 0.19 |
| . | . | CebpaCre/+ LSK cells (EYFP+ vs EYFP−) . | Cebpanull LSK cells (EYFP+ vs EYFP−) . |
|---|---|---|---|
| Up-regulation | |||
| S100a8 | S-100 calcium-binding protein A8 | 55.8 | 0.76 |
| Ear2 | Eosinophil-associated ribonuclease A | 32.4 | 3.2 |
| Ly6c1 | Ly6c antigen, locus C1 | 21.6 | 0.83 |
| Ctsg | Cathepsin G | 14.5 | 1.8 |
| Ccr2 | Chemokine receptor 2 | 8.6 | 1.3 |
| Fcgr3 | Fc receptor, IgG, high-affinity III | 8.0 | 2.0 |
| Irf8 | Interferon regulatory factor 8 | 6.6 | 0.72 |
| Vcam1 | Vascular cell adhesion molecule 1 | 5.8 | 1.2 |
| Mpo | Myeloperoxidase | 5.2 | 1.0 |
| Csf1r | M-CSF receptor | 5.1 | 1.6 |
| Id2 | Inhibitor of DNA binding 2 | 4.4 | 1.4 |
| Csfr2b | GM-CSF receptor, subunit b | 2.5 | 0.45 |
| Lyz1 | Lysozyme 1 | 1.9 | 0.53 |
| Down-regulation | |||
| Evi1 | Ectropic viral integration site 1 | 0.18 | 0.03 |
| Ndn | Necdin | 0.21 | 0.4 |
| Rag1 | Recombination activating gene 1 | 0.23 | 1.5 |
| Ebf1 | Early B-cell factor 1 | 0.28 | 2.4 |
| Procr | Protein C receptor, endothelial | 0.29 | 0.23 |
| Mll5 | Mixed-lineage leukemia 5 | 0.35 | 1.76 |
| Vpreb1 | Pre-B lymphocyte gene 1 | 0.36 | 0.94 |
| Hoxa9 | Homeobox A9 | 0.38 | 0.65 |
| Pik3cd | PI3-kinase catalytic delta polypeptide | 0.39 | 1.27 |
| Foxo3 | Forkhead box O3 | 0.40 | 0.84 |
| Hoxa5 | Homeobox A5 | 0.41 | 0.83 |
| Tcf4 | Transcription factor 4 | 0.43 | 1.68 |
| Mycn | v-myc related, neuroblastoma derived | 0.48 | 0.34 |
| Ccr9 | Chemokine receptor 9 | 0.50 | 0.19 |
Microarray analysis of the expression of genes involved in the biology of stem cells, myeloid and lymphoid cells, presented as “fold-change” in EYFP+ bone marrow LSK cells of Cebpacre/+ R26 EYFP mice compared with EYFP− LSK cells (third column). Data represent the mean “fold change” obtained from expression levels of two (EYFP−) and three (EYFP+) independent experiments. In the fourth column, the fold change in expression of the same probe sets/genes obtained from EYFP+ vs EYFP− LSK cells of fetal livers of Cebpacre/Fl R26 EYFP embryos is given (mean “fold change” obtained from expression levels of 2 independent experiments).