Inhibition of BFU-E and CFU-E colony formation by HZ and HNE
| . | CFU-E . | BFU-E . | CFU-GM . | |||
|---|---|---|---|---|---|---|
| Mean (SE) . | n . | Mean (SE) . | n . | Mean (SE) . | n . | |
| Day 4 | ||||||
| HZ | 59.2* (15.4) | 5 | 54.6* (14.3) | 5 | 76.2 (10.5) | 5 |
| HNE | 51.3* (12.0) | 5 | 68.2* (10.4) | 5 | 84.6 (8.1) | 5 |
| Day 8 | ||||||
| HZ | 51.4* (8.1) | 4 | 51.8* (18.7) | 4 | 43.2* (5.8) | 4 |
| HNE | 37.3* (12.8) | 4 | 53.7* (18.1) | 4 | 61.8* (11.4) | 4 |
| Day 14 | ||||||
| HZ | 35.8* (6.3) | 5 | no colonies | 5 | no colonies | 5 |
| HNE | 45.9* (28.6) | 5 | no colonies | 5 | no colonies | 5 |
| . | CFU-E . | BFU-E . | CFU-GM . | |||
|---|---|---|---|---|---|---|
| Mean (SE) . | n . | Mean (SE) . | n . | Mean (SE) . | n . | |
| Day 4 | ||||||
| HZ | 59.2* (15.4) | 5 | 54.6* (14.3) | 5 | 76.2 (10.5) | 5 |
| HNE | 51.3* (12.0) | 5 | 68.2* (10.4) | 5 | 84.6 (8.1) | 5 |
| Day 8 | ||||||
| HZ | 51.4* (8.1) | 4 | 51.8* (18.7) | 4 | 43.2* (5.8) | 4 |
| HNE | 37.3* (12.8) | 4 | 53.7* (18.1) | 4 | 61.8* (11.4) | 4 |
| Day 14 | ||||||
| HZ | 35.8* (6.3) | 5 | no colonies | 5 | no colonies | 5 |
| HNE | 45.9* (28.6) | 5 | no colonies | 5 | no colonies | 5 |
Growth inhibition in cultures supplemented with HZ and HNE is shown as percentage of control cell growth. Erythroid cells were cocultivated with HZ (25μM HZ-heme) or treated with HNE (7μM final concentration) or kept as untreated controls. At indicated times, 103 cells were seeded in semisolid medium to assess their colony-forming ability.
Significant difference (P < .05) between HZ-treated or HNE-treated cells and controls.