Primers for TAC4 genotyping, generation of probes, and gene expression analysis
| Probe/primer . | Sequence, 5′-3′ . |
|---|---|
| Genotyping PCR | |
| TAC4 forward | CCTGGGGTAAACTAGAATGGT |
| WT reverse | GTACCAGAGCTAAGGGCTGTTC |
| neo reverse | CATCGCCTTCTATCGCCTTCT |
| Southern probe neo | |
| neo2 forward | GAAGAACTCGTCAAGAAGGCG |
| neo1 reverse | AACAAGATGGATTGCACGCAG |
| Southern probe TAC4 | |
| HK1 ex1 forward | TCCCATGAAGCATGGCAAAG |
| HK1 ex1 reverse | ACCAAAAGCCAGTTCCTCT |
| RT-PCR β-actin | |
| β-Actin forward | TCCCTGGAGAAGAGCTACGA |
| β-Actin reverse | ATCTGCTGGAAGGTGGACAG |
| RT-PCR TAC4 | |
| TAC4 forward | CGGGCCATCAGTGTGCACTA |
| TAC4 711 reverse | CGTTGCTACCAGATGCCAAGAG |
| Northern probe TAC4 | |
| TAC4 CM2 forward | GAGGAGATGTGACAGGCAGGTG |
| TAC4 genA1 reverse | ACATATGCCATGACACATGCAGAG |
| Northern probe L32 | |
| L32 forward | GTGAAGCCCAAGATCGTC |
| L32 reverse | AGCAATCTCAGCACAGTAAG |
| Global RT-PCR TAC4 | |
| 3′ forward 2 TAC4 | GAAGACGCTGCATGTATTG |
| 3′ reverse TAC4 | CATATGCCATGACACATGCAG |
| Global RT-PCR NK-1 | |
| 3′ forward TACR1 | CTCCAGTATCGACAGGCTGG |
| 3′ reverse TACR1 | ACAGATGCTTCAGGAATGAC |
| Probe/primer . | Sequence, 5′-3′ . |
|---|---|
| Genotyping PCR | |
| TAC4 forward | CCTGGGGTAAACTAGAATGGT |
| WT reverse | GTACCAGAGCTAAGGGCTGTTC |
| neo reverse | CATCGCCTTCTATCGCCTTCT |
| Southern probe neo | |
| neo2 forward | GAAGAACTCGTCAAGAAGGCG |
| neo1 reverse | AACAAGATGGATTGCACGCAG |
| Southern probe TAC4 | |
| HK1 ex1 forward | TCCCATGAAGCATGGCAAAG |
| HK1 ex1 reverse | ACCAAAAGCCAGTTCCTCT |
| RT-PCR β-actin | |
| β-Actin forward | TCCCTGGAGAAGAGCTACGA |
| β-Actin reverse | ATCTGCTGGAAGGTGGACAG |
| RT-PCR TAC4 | |
| TAC4 forward | CGGGCCATCAGTGTGCACTA |
| TAC4 711 reverse | CGTTGCTACCAGATGCCAAGAG |
| Northern probe TAC4 | |
| TAC4 CM2 forward | GAGGAGATGTGACAGGCAGGTG |
| TAC4 genA1 reverse | ACATATGCCATGACACATGCAGAG |
| Northern probe L32 | |
| L32 forward | GTGAAGCCCAAGATCGTC |
| L32 reverse | AGCAATCTCAGCACAGTAAG |
| Global RT-PCR TAC4 | |
| 3′ forward 2 TAC4 | GAAGACGCTGCATGTATTG |
| 3′ reverse TAC4 | CATATGCCATGACACATGCAG |
| Global RT-PCR NK-1 | |
| 3′ forward TACR1 | CTCCAGTATCGACAGGCTGG |
| 3′ reverse TACR1 | ACAGATGCTTCAGGAATGAC |