Table 3 Clonal TCR rearrangements in... | American Society of Hematology

Table 3

Clonal TCR rearrangements in patients treated with dasatinib (n = 20)

Patient no.	Immunophenotype of LGL cells	TCR δ primer pairs						TCR γ primer pairs
Patient no.	Immunophenotype of LGL cells	1	2	3	4	5	6	1	2	3	4	5	6	7	8	9	10	11	12
1	T and NK cell		X													X
2	T cell	X		X	X				X		X		X
3	T cell			X													X
4	NK cell		X					X						X
5	T and NK cell					X					X					X
6	NK cell		X
7	T and NK cell		X																X
8	T cell							X				X
9	T cell		X						X							X
10	NK cell		X					X
11									X		X	X
12												X
13																X
14			X					X										X
15
16
17											X		X
18													X				X
19
20																X

Patient no.	Immunophenotype of LGL cells	TCR δ primer pairs						TCR γ primer pairs
Patient no.	Immunophenotype of LGL cells	1	2	3	4	5	6	1	2	3	4	5	6	7	8	9	10	11	12
1	T and NK cell		X													X
2	T cell	X		X	X				X		X		X
3	T cell			X													X
4	NK cell		X					X						X
5	T and NK cell					X					X					X
6	NK cell		X
7	T and NK cell		X																X
8	T cell							X				X
9	T cell		X						X							X
10	NK cell		X					X
11									X		X	X
12												X
13																X
14			X					X										X
15
16
17											X		X
18													X				X
19
20																X

Patients 1 to 10 had persistent lymphocytosis with large granular lymphocyte (LGL) morphology during dasatinib treatment. In patients 11 to 20 lymphocyte counts were normal during therapy. Clonality of the lymphocytes was determined by PCR and gel analysis with the use of 12 primer pairs for the TCR γ gene rearrangements and 6 primer pairs for TCR δ gene rearrangements. Positive clonal PCR products were confirmed with the sequencing and are marked in the columns as X.

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