Table 4 Quantitative follow-up and FACS... | American Society of Hematology

Table 4

Quantitative follow-up and FACS sorting of lymphocytes with individual allele-specific oligonucleotide (ASO) primer

Patient no.	LGL lymphocytosis	Gene	Quantitative follow-up	Lymphocyte population	Cell-surface TCR expression
1	Yes	Incomplete TCRD	Yes	NK cell	NA
1	Yes	Complete TCRG	Yes	CD8⁺ T cell	TCR αβ⁺
2	Yes	Incomplete TCRD	Yes	CD8⁺ T cell	ND
2	Yes	Complete TCRD	Yes	CD8⁺ T cell	TCR γδ⁺
2	Yes	Complete TCRG	Yes	CD4⁺ T cell	TCR αβ⁺
2	Yes	Complete TCRG	Yes	CD4⁺ T cell	ND
4	Yes	Incomplete TCRD	No	NK cell	NA
5	Yes	Incomplete TCRD	Yes	CD4⁺ T cell	TCR γδ⁺
5	Yes	Complete TCRG	Yes	CD4⁺ T cell	TCR αβ⁺
6	Yes	Incomplete TCRD	No	NK cell	NA
7	Yes	Incomplete TCRD	No	NK cell	NA
8	Yes	Complete TCRG	Yes	CD8⁺ T cell	ND
10	Yes	Incomplete TCRD	No	ND	ND
10	Yes	Complete TCRG	No	CD8⁺ T cell	ND
12	No	Complete TCRG	Yes	ND	ND
17	No	Complete TCRG	No	CD8⁺ T cell	ND
18	No	Complete TCRG	Yes	CD8⁺ T cell	ND
18	No	Complete TCRG	Yes	CD8⁺ T cell	ND

Patient no.	LGL lymphocytosis	Gene	Quantitative follow-up	Lymphocyte population	Cell-surface TCR expression
1	Yes	Incomplete TCRD	Yes	NK cell	NA
1	Yes	Complete TCRG	Yes	CD8⁺ T cell	TCR αβ⁺
2	Yes	Incomplete TCRD	Yes	CD8⁺ T cell	ND
2	Yes	Complete TCRD	Yes	CD8⁺ T cell	TCR γδ⁺
2	Yes	Complete TCRG	Yes	CD4⁺ T cell	TCR αβ⁺
2	Yes	Complete TCRG	Yes	CD4⁺ T cell	ND
4	Yes	Incomplete TCRD	No	NK cell	NA
5	Yes	Incomplete TCRD	Yes	CD4⁺ T cell	TCR γδ⁺
5	Yes	Complete TCRG	Yes	CD4⁺ T cell	TCR αβ⁺
6	Yes	Incomplete TCRD	No	NK cell	NA
7	Yes	Incomplete TCRD	No	NK cell	NA
8	Yes	Complete TCRG	Yes	CD8⁺ T cell	ND
10	Yes	Incomplete TCRD	No	ND	ND
10	Yes	Complete TCRG	No	CD8⁺ T cell	ND
12	No	Complete TCRG	Yes	ND	ND
17	No	Complete TCRG	No	CD8⁺ T cell	ND
18	No	Complete TCRG	Yes	CD8⁺ T cell	ND
18	No	Complete TCRG	Yes	CD8⁺ T cell	ND

To quantify the amount of lymphocyte clone in correlation with the lymphocyte count and to identify the lymphocyte fraction where clonal cells reside, patient-specific ASO primers were designed for 11 patients. Patients 1 to 10 had persistent lymphocytosis with large granular lymphocyte (LGL) morphology during dasatinib treatment. In patients 12, 17, and 18, lymphocyte counts were normal during the therapy. No sorting was done from patient 12 because of small number of cells.

NK cell indicates CD3^neg CD16/56⁺; CD8⁺ T cell, CD3⁺CD8⁺; CD4⁺ T cell, CD3⁺CD8^neg; ND, not done; and NA, not applicable.

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