Sequences of clonal TCR δ-gene rearrangements detected in LGLpos patients
| Patient no. . | Clonal cell population . | Vδ2/Vδ3 . | N1 . | Dδ1 . | N2 . | Dδ2 . | N3 . | Dδ3 . | N4 . | Jδ . | Function of rearrangement and junction region amino acids (complete and productive rearrangements) . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Germline | Vδ2 tgtgcctgtgacacc Vδ3 ctactgtgcctttag | gaaatagt | ccttcctac | actgggggatacg | acaccgataaactcatc | ||||||
| 1 | NK cell | Vδ2 tgtgcctgt | attt | actgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 2 | CD8+ | Vδ2 tgtgcctgtgacacc | gaagac | ctgggggata | aggctc | caccgataaactcatc | Unproductive (stop codons, out-of-frame junction) | ||||
| 2 | TCR γδ+ CD8+ | Vδ3 ctactgtgccttta | atag | cgt | ttcctac | ggggtttg | gggggata | atgg | cgataaactcatc | Productive CAFNSVSYGVWGDNGDKLIF | |
| 3 | NA | Vδ2 tgtgcctgtgacacc | gtatt | actgggggatacg | cggcg | caccgataaactcatc | Productive CACDTVLLGDTRRTDKLIF | ||||
| 4 | NK cell | Vδ2 tgtgcctgtgac | ttcttcccttttg | ctgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 5 | TCR γδ+ CD4+ | ccttccta | actggagggg | accgataaactcatc | Incomplete (no V gene); unproductive | ||||||
| 6 | NK cell | Vδ2 tgtgcctgtgacacc | gtgt | tgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 7 | NK cell | Vδ2 tgtgcctgtgacac | gggct | actgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 9 | NA | Vδ2 tgtgcctgt | tctgtacccgt | actgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 10 | NA | Vδ2 tgtgcctgtgac | cgtt | actgggggatacg | Incomplete (no J gene); unproductive |
| Patient no. . | Clonal cell population . | Vδ2/Vδ3 . | N1 . | Dδ1 . | N2 . | Dδ2 . | N3 . | Dδ3 . | N4 . | Jδ . | Function of rearrangement and junction region amino acids (complete and productive rearrangements) . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Germline | Vδ2 tgtgcctgtgacacc Vδ3 ctactgtgcctttag | gaaatagt | ccttcctac | actgggggatacg | acaccgataaactcatc | ||||||
| 1 | NK cell | Vδ2 tgtgcctgt | attt | actgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 2 | CD8+ | Vδ2 tgtgcctgtgacacc | gaagac | ctgggggata | aggctc | caccgataaactcatc | Unproductive (stop codons, out-of-frame junction) | ||||
| 2 | TCR γδ+ CD8+ | Vδ3 ctactgtgccttta | atag | cgt | ttcctac | ggggtttg | gggggata | atgg | cgataaactcatc | Productive CAFNSVSYGVWGDNGDKLIF | |
| 3 | NA | Vδ2 tgtgcctgtgacacc | gtatt | actgggggatacg | cggcg | caccgataaactcatc | Productive CACDTVLLGDTRRTDKLIF | ||||
| 4 | NK cell | Vδ2 tgtgcctgtgac | ttcttcccttttg | ctgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 5 | TCR γδ+ CD4+ | ccttccta | actggagggg | accgataaactcatc | Incomplete (no V gene); unproductive | ||||||
| 6 | NK cell | Vδ2 tgtgcctgtgacacc | gtgt | tgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 7 | NK cell | Vδ2 tgtgcctgtgacac | gggct | actgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 9 | NA | Vδ2 tgtgcctgt | tctgtacccgt | actgggggatacg | Incomplete (no J gene); unproductive | ||||||
| 10 | NA | Vδ2 tgtgcctgtgac | cgtt | actgggggatacg | Incomplete (no J gene); unproductive |
The table represents detected TCR δ rearrangements. Sequences of the junction region (VDJ) are aligned.