Table 3 Oligonucleotide primers for PCR... | American Society of Hematology

Table 3

Oligonucleotide primers for PCR amplification of genomic DNA prepared from persons with B_m and AB_m, as well as the common ABO types and other B subgroups

Primer*	Sequence (5′ → 3′)	PCR†
ABO−186S	5′ −GCGCCGTCCCTTCCTAGCAG−3′	6
ABO+3043S	5′ −GGAACTCCTCTGCAGTCATTCCC−3′	1, 2
ABO+10803S	5′ −TGTTTACAGAAGCTTTGTTGCTAGTGGCCC−3′	3
ABO+4419S	5′ −TGGAATTGCTGTCTCCTCTTTTAGTCC−3′	4, 5, 7
ABO+11044AS	5′ −TCCCTGGCGTTTCCCATACAC−3′	6
ABO+11078AS	5′ −GGTCCCTCCTGACCCTGACAA−3′	4
ABO+12797AS	5′ −ATCAGCCCGACTGCTCCTGCAGACTCT−3′	2
ABO+15374AS	5′ −GAGACATGCTGCAGATGGTCAGG−3′	1, 3
ABO+17909AS	5′ −CGCCACCCCACTCTGCTTG−3′	5
ABO+19915AS	5′ −GGCGTATCTGCGATTGCGTGT−3′	7
ABO+17621S	5′ −GAATTCATGTGGGTGGCACCCTGCCA−3′	8
ABO+17860AS	5′ −GAATTCACTCGCCACTGCCTGGGTCTC−3′	8
ABO+18812S	5′ −GGATCCAGGGGTGCACGGCCGGCGGC−3′	9
ABO+19109AS	5′ −AGGTAATCCACCTCGCTGAGGAA−3′	9

Primer*	Sequence (5′ → 3′)	PCR†
ABO−186S	5′ −GCGCCGTCCCTTCCTAGCAG−3′	6
ABO+3043S	5′ −GGAACTCCTCTGCAGTCATTCCC−3′	1, 2
ABO+10803S	5′ −TGTTTACAGAAGCTTTGTTGCTAGTGGCCC−3′	3
ABO+4419S	5′ −TGGAATTGCTGTCTCCTCTTTTAGTCC−3′	4, 5, 7
ABO+11044AS	5′ −TCCCTGGCGTTTCCCATACAC−3′	6
ABO+11078AS	5′ −GGTCCCTCCTGACCCTGACAA−3′	4
ABO+12797AS	5′ −ATCAGCCCGACTGCTCCTGCAGACTCT−3′	2
ABO+15374AS	5′ −GAGACATGCTGCAGATGGTCAGG−3′	1, 3
ABO+17909AS	5′ −CGCCACCCCACTCTGCTTG−3′	5
ABO+19915AS	5′ −GGCGTATCTGCGATTGCGTGT−3′	7
ABO+17621S	5′ −GAATTCATGTGGGTGGCACCCTGCCA−3′	8
ABO+17860AS	5′ −GAATTCACTCGCCACTGCCTGGGTCTC−3′	8
ABO+18812S	5′ −GGATCCAGGGGTGCACGGCCGGCGGC−3′	9
ABO+19109AS	5′ −AGGTAATCCACCTCGCTGAGGAA−3′	9

Numerals of primers represent locations of the 5′terminus relative to the ATG translation start site of exon 1 in ABO on genomic DNA of NCBI reference sequence NT_035014.4.

†

PCR amplification in which the primer was used is indicated.

View Large

This Feature Is Available To Subscribers Only