Table 1 PCR primers used for CCR7... | American Society of Hematology

Table 1

PCR primers used for CCR7 cloning and RT-PCR analysis

	Primer sequence	Band size, bp
CCR7: cloning primers	Forward: 5′-aatatcgatagccgccaccatggacctggg-3′	1167*
CCR7: cloning primers	Reverse: 5′-atgactagtagagtcgcctatgg-3′	1167*
CCR7: transgene RT-PCR primers	Forward: 5′-atggacctggggaaaccactgaaaagc-3′	398^†
CCR7: transgene RT-PCR primers	Reverse: 5′-ctggagcagggtgcggatgatgac-3′	398^†
CCR7: endogenous RT-PCR primers	Forward: 5′-gcaacgatctcttcaagctcttcaagga-3′	214†
CCR7: endogenous RT-PCR primers	Reverse: 5′-tgagtcattgcatctgctccctatccc-3′	214†
GAPDH: RT-PCR primers	Forward: 5′-atggggaaggtgaaggtcggagtcaa-3′	577^†
GAPDH: RT-PCR primers	Reverse: 5′-cggaggggccatccacagtcttct-3′	577^†

	Primer sequence	Band size, bp
CCR7: cloning primers	Forward: 5′-aatatcgatagccgccaccatggacctggg-3′	1167*
CCR7: cloning primers	Reverse: 5′-atgactagtagagtcgcctatgg-3′	1167*
CCR7: transgene RT-PCR primers	Forward: 5′-atggacctggggaaaccactgaaaagc-3′	398^†
CCR7: transgene RT-PCR primers	Reverse: 5′-ctggagcagggtgcggatgatgac-3′	398^†
CCR7: endogenous RT-PCR primers	Forward: 5′-gcaacgatctcttcaagctcttcaagga-3′	214†
CCR7: endogenous RT-PCR primers	Reverse: 5′-tgagtcattgcatctgctccctatccc-3′	214†
GAPDH: RT-PCR primers	Forward: 5′-atggggaaggtgaaggtcggagtcaa-3′	577^†
GAPDH: RT-PCR primers	Reverse: 5′-cggaggggccatccacagtcttct-3′	577^†

PCR program: 95°C for 5 minutes, 95°C for 30 seconds, 60°C for 30 seconds, 72°C for 1.5 minutes for 35 cycles; 72°C for 10 minutes, and 4°C.

PCR program: 95°C for 5 minutes, 95°C for 30 seconds, 65°C for 30 seconds, and 72°C for 45 seconds, 30 cycles.