Transcription factors with altered DNA binding in cell lines with manipulated SOD2 expression levels
| Transcription factor . | Fold change SOD2 . | Fold change shSOD2 . |
|---|---|---|
| With decreased DNA binding upon infection with SOD2 retrovirus . | ||
| EKLF | −174.13 | 1.87 |
| EGFBP | −33.25 | 2.61 |
| NF-Atp | −10.67 | 1.98 |
| c-ets-1 | −10.09 | 1.13 |
| XBP1 | −8.73 | 1.10 |
| RSRFC4 (Ras-responsive element) | −7.25 | 1.42 |
| NFIL-2 (IL-2-binding element) | −6.63 | 1.31 |
| NFATc | −4.55 | 4.66 |
| ACPBP | −4.23 | 1.68 |
| KTP1 | −3.07 | 5.50 |
| TCE | −2.79 | 1.47 |
| HiNF-B/H1 | −2.54 | 4.95 |
| WT1 | −2.38 | 1.08 |
| Isl-1 | −1.73 | 1.11 |
| PYR | −1.62 | 1.45 |
| NRF-1 | −0.28 | 8.20 |
| EGR2BP | Undetectable | 273.75 |
| GAS | Undetectable | 21.66 |
| With increased DNA-binding upon infection with SOD2 retrovirus | ||
| KPF1 | 31.28 | Undetectable |
| Tf-LF | 12.62 | −44.28 |
| Freac-2 (FoxF2) | 9.01 | Undetectable |
| Freac-7 (FoxL1) | 8.02 | −2.02 |
| IRF-1 | 4.57 | −2.33 |
| DEI (rat albumin-binding element) | 3.35 | −8.43 |
| HiNF-A | 2.50 | −10.06 |
| Ikaros | 2.32 | −1.16 |
| COUP-TF | 1.92 | −1.14 |
| NPAS2 | 1.67 | −1.01 |
| CREB2 | 1.66 | −1.36 |
| cyp1a1 | 1.49 | −1.26 |
| CdxA/NKX | 1.27 | −4.86 |
| Transcription factor . | Fold change SOD2 . | Fold change shSOD2 . |
|---|---|---|
| With decreased DNA binding upon infection with SOD2 retrovirus . | ||
| EKLF | −174.13 | 1.87 |
| EGFBP | −33.25 | 2.61 |
| NF-Atp | −10.67 | 1.98 |
| c-ets-1 | −10.09 | 1.13 |
| XBP1 | −8.73 | 1.10 |
| RSRFC4 (Ras-responsive element) | −7.25 | 1.42 |
| NFIL-2 (IL-2-binding element) | −6.63 | 1.31 |
| NFATc | −4.55 | 4.66 |
| ACPBP | −4.23 | 1.68 |
| KTP1 | −3.07 | 5.50 |
| TCE | −2.79 | 1.47 |
| HiNF-B/H1 | −2.54 | 4.95 |
| WT1 | −2.38 | 1.08 |
| Isl-1 | −1.73 | 1.11 |
| PYR | −1.62 | 1.45 |
| NRF-1 | −0.28 | 8.20 |
| EGR2BP | Undetectable | 273.75 |
| GAS | Undetectable | 21.66 |
| With increased DNA-binding upon infection with SOD2 retrovirus | ||
| KPF1 | 31.28 | Undetectable |
| Tf-LF | 12.62 | −44.28 |
| Freac-2 (FoxF2) | 9.01 | Undetectable |
| Freac-7 (FoxL1) | 8.02 | −2.02 |
| IRF-1 | 4.57 | −2.33 |
| DEI (rat albumin-binding element) | 3.35 | −8.43 |
| HiNF-A | 2.50 | −10.06 |
| Ikaros | 2.32 | −1.16 |
| COUP-TF | 1.92 | −1.14 |
| NPAS2 | 1.67 | −1.01 |
| CREB2 | 1.66 | −1.36 |
| cyp1a1 | 1.49 | −1.26 |
| CdxA/NKX | 1.27 | −4.86 |
Transcriptional binding activity of 354 transcription factors were determined by Panomic's DNA/protein array in OPM-2 transduced with either a control retrovirus, a SOD2 retrovirus or an shSOD2 retrovirus. Changes in intensity of spots were determined by densitometry. All transcription factors showing a reciprocal modulation in binding activity with the SOD2 overexpression and the shSOD2 array are shown, and their fold change respective to the control retrovirus is shown. The experiment was performed twice, and a representative experiment is shown.