Proteins identified by mass spectrometry in the anti-LRRFIP1 pull-down from resting and activated platelets
| IPI ID . | HGNC gene ID* . | Description . | No. of peptides present . | |||
|---|---|---|---|---|---|---|
| Xtandem . | Sequest . | |||||
| Resting . | Activated . | Resting . | Activated . | |||
| IPI00478003.1 | A2M | Alpha-2-macroglobulin | 2 | 1 | 1 | 1 |
| IPI00297550.8 | F13A1 | Coagulation factor xiiia | 2 | 6 | 3 | 4 |
| IPI00021458.1 | EHD3 | eh domain-containing protein 3 | 1 | 2 | 0 | 2 |
| IPI00513782.3 | GSN | Gelsolin | 4 | 13 | 0 | 0 |
| IPI00026272.2 | HIST1H2AB/HIST1H2AE | Histone h2a type 1-b | 2 | 1 | 2 | 3 |
| IPI00003406.3 | DBN1 | Isoform 1 of drebrin | 14 | 33 | 11 | 35 |
| IPI00017030.10 | MYO18A | Isoform 1 of myosin-xviiia | 15 | 6 | 16 | 4 |
| IPI00180404.7 | NEXN | Isoform 1 of nexilin | 4 | 16 | 3 | 12 |
| IPI00014898.2 | PLEC1 | Isoform 1 of plectin-1 | 1 | 15 | 1 | 12 |
| IPI00006207.2 | LRRFIP1 | Leucine-rich repeat flightless-interacting protein 1 | 1 | 4 | 0 | 5 |
| IPI00220278.5 | MYL9 | Myosin regulatory light chain2; smooth muscle | 3 | 5 | 4 | 7 |
| IPI00031023.1 | FLII | Protein flightless-1 homolog | 16 | 26 | 17 | 21 |
| IPI00005087.1 | TMOD3 | Tropomodulin-3 | 5 | 13 | 6 | 8 |
| IPI00176593.3 | No symbol | Uncharacterized protein ensp00000380341 | 4 | 10 | 8 | 11 |
| IPI00298971.1 | VTN | Vitronectin precursor | 3 | 2 | 1 | 1 |
| IPI00218474.5 | ENO3 | Beta-enolase | 5 | 0 | 3 | 0 |
| IPI00399257.2 | DCHS2 | CDNA flj45804 fis | 2 | 0 | 0 | 0 |
| IPI00027487.3 | CKM | Creatine kinase m-type | 2 | 0 | 3 | 0 |
| IPI00218130.3 | PYGM | Glycogen phosphorylase; muscle form | 3 | 0 | 4 | 0 |
| IPI00024804.3 | ATP2A1 | Serca1b of sarcoplasmic | 2 | 0 | 2 | 0 |
| IPI00000105.4 | MVP | Major vault protein | 4 | 0 | 1 | 0 |
| IPI00001753.1 | MYH4 | Myosin-4 | 8 | 0 | 6 | 0 |
| IPI00383071.1 | TPI1 | Triosephosphate isomerase (fragment) | 2 | 0 | 2 | 0 |
| IPI00183968.4 | TPM3 | Tropomyosin 3 isoform 1 | 3 | 0 | 3 | 0 |
| IPI00023014.1 | VWF | Von willebrand factor | 2 | 0 | 2 | 0 |
| IPI00021536.1 | CALML5 | Calmodulin-like protein 5 | 0 | 1 | 0 | 3 |
| IPI00007425.1 | DSC1 | Desmocollin 1 | 0 | 2 | 0 | 2 |
| IPI00010951.2 | EPPK1 | Epiplakin | 0 | 5 | 0 | 7 |
| IPI00025512.2 | HSPB1 | Heat shock protein beta-1 | 0 | 1 | 0 | 2 |
| IPI00013890.2 | SFN | 14-3-3 protein sigma | 0 | 1 | 0 | 3 |
| IPI00550792.4 | BIN2 | Bridging integrator 2 | 0 | 2 | 0 | 3 |
| IPI00007277.2 | LRRFIP2 | Leucine-rich repeat flightless-interacting protein 2 | 0 | 2 | 0 | 3 |
| IPI00299048.6 | IQGAP2 | ras GTPase-activating-like protein iqgap2 | 0 | 2 | 0 | 3 |
| IPI00071509.1 | PKP1 | Plakophilin-1 | 0 | 3 | 0 | 3 |
| IPI00013933.2 | DSP | DPI of desmoplakin | 0 | 28 | 0 | 24 |
| IPI00045550.4 | PPP1R9B | Protein phosphatise-1; regulatory subunit 9b | 0 | 3 | 0 | 0 |
| IPI00033583.3 | SERPINB12 | Serpin b12 | 0 | 2 | 0 | 3 |
| IPI ID . | HGNC gene ID* . | Description . | No. of peptides present . | |||
|---|---|---|---|---|---|---|
| Xtandem . | Sequest . | |||||
| Resting . | Activated . | Resting . | Activated . | |||
| IPI00478003.1 | A2M | Alpha-2-macroglobulin | 2 | 1 | 1 | 1 |
| IPI00297550.8 | F13A1 | Coagulation factor xiiia | 2 | 6 | 3 | 4 |
| IPI00021458.1 | EHD3 | eh domain-containing protein 3 | 1 | 2 | 0 | 2 |
| IPI00513782.3 | GSN | Gelsolin | 4 | 13 | 0 | 0 |
| IPI00026272.2 | HIST1H2AB/HIST1H2AE | Histone h2a type 1-b | 2 | 1 | 2 | 3 |
| IPI00003406.3 | DBN1 | Isoform 1 of drebrin | 14 | 33 | 11 | 35 |
| IPI00017030.10 | MYO18A | Isoform 1 of myosin-xviiia | 15 | 6 | 16 | 4 |
| IPI00180404.7 | NEXN | Isoform 1 of nexilin | 4 | 16 | 3 | 12 |
| IPI00014898.2 | PLEC1 | Isoform 1 of plectin-1 | 1 | 15 | 1 | 12 |
| IPI00006207.2 | LRRFIP1 | Leucine-rich repeat flightless-interacting protein 1 | 1 | 4 | 0 | 5 |
| IPI00220278.5 | MYL9 | Myosin regulatory light chain2; smooth muscle | 3 | 5 | 4 | 7 |
| IPI00031023.1 | FLII | Protein flightless-1 homolog | 16 | 26 | 17 | 21 |
| IPI00005087.1 | TMOD3 | Tropomodulin-3 | 5 | 13 | 6 | 8 |
| IPI00176593.3 | No symbol | Uncharacterized protein ensp00000380341 | 4 | 10 | 8 | 11 |
| IPI00298971.1 | VTN | Vitronectin precursor | 3 | 2 | 1 | 1 |
| IPI00218474.5 | ENO3 | Beta-enolase | 5 | 0 | 3 | 0 |
| IPI00399257.2 | DCHS2 | CDNA flj45804 fis | 2 | 0 | 0 | 0 |
| IPI00027487.3 | CKM | Creatine kinase m-type | 2 | 0 | 3 | 0 |
| IPI00218130.3 | PYGM | Glycogen phosphorylase; muscle form | 3 | 0 | 4 | 0 |
| IPI00024804.3 | ATP2A1 | Serca1b of sarcoplasmic | 2 | 0 | 2 | 0 |
| IPI00000105.4 | MVP | Major vault protein | 4 | 0 | 1 | 0 |
| IPI00001753.1 | MYH4 | Myosin-4 | 8 | 0 | 6 | 0 |
| IPI00383071.1 | TPI1 | Triosephosphate isomerase (fragment) | 2 | 0 | 2 | 0 |
| IPI00183968.4 | TPM3 | Tropomyosin 3 isoform 1 | 3 | 0 | 3 | 0 |
| IPI00023014.1 | VWF | Von willebrand factor | 2 | 0 | 2 | 0 |
| IPI00021536.1 | CALML5 | Calmodulin-like protein 5 | 0 | 1 | 0 | 3 |
| IPI00007425.1 | DSC1 | Desmocollin 1 | 0 | 2 | 0 | 2 |
| IPI00010951.2 | EPPK1 | Epiplakin | 0 | 5 | 0 | 7 |
| IPI00025512.2 | HSPB1 | Heat shock protein beta-1 | 0 | 1 | 0 | 2 |
| IPI00013890.2 | SFN | 14-3-3 protein sigma | 0 | 1 | 0 | 3 |
| IPI00550792.4 | BIN2 | Bridging integrator 2 | 0 | 2 | 0 | 3 |
| IPI00007277.2 | LRRFIP2 | Leucine-rich repeat flightless-interacting protein 2 | 0 | 2 | 0 | 3 |
| IPI00299048.6 | IQGAP2 | ras GTPase-activating-like protein iqgap2 | 0 | 2 | 0 | 3 |
| IPI00071509.1 | PKP1 | Plakophilin-1 | 0 | 3 | 0 | 3 |
| IPI00013933.2 | DSP | DPI of desmoplakin | 0 | 28 | 0 | 24 |
| IPI00045550.4 | PPP1R9B | Protein phosphatise-1; regulatory subunit 9b | 0 | 3 | 0 | 0 |
| IPI00033583.3 | SERPINB12 | Serpin b12 | 0 | 2 | 0 | 3 |
The number of peptides is a semiquantitative measure of protein abundance. This experimental information has been used to create the LRRFIP1 interaction network, as shown in Figure 6B. List of the International Protein Index identifiers (IPI ID) based on the peptides that have been detected by mass spectrometry in the anti-LRRFIP1 pull-downs obtained from platelets.
Gene identifier (ID) is by the HUGO Gene Nomenclature Committee (HGNC). Proteins shown in bold were validated by Western blotting.