Comparison of complete blood counts and frequency of EGFP+ HSC/Ps and EGFP+ B-lymphoid progenitors present in the BM in mice
| Primary recipient group . | WBCs, × 103/mm3 . | ANC, × 103/mm3 . | PLTs, × 103/mm3 . | Hb, g/dL . | Spleen weight, mg . | EGFP+ LT-HSCs, % . | EGFP+ ST-HSCs, % . | EGFP+ MPPs, % . | EGFP+ B220lo, % . |
|---|---|---|---|---|---|---|---|---|---|
| Ntg;MOCK | 10.6 ± 1.9 | 2.7 ± 0.6 | 940.3 ± 101.3 | 14.4 ± 0.7 | 40 ± 10 | 0.031 ± 0.003 | 0.099 ± 0.066 | 0.098 ± 0.063 | 0.34 ± 0.10 |
| Ntg;Bmi1 | 8.7 ± 2.3 | 2.5 ± 0.7 | 931.0 ± 181.4 | 13.0 ± 1.6 | 40 ± 5 | 0.021 ± 0.003 | 0.106 ± 0.070 | 0.118 ± 0.068 | 0.42 ± 0.05 |
| Scl/p210;MOCK | 12.6 ± 3.6 | 2.7 ± 0.7 | 999.8 ± 90.8 | 13.0 ± 0.6 | 49 ± 13 | 0.038 ± 0.005 | 0.106 ± 0.108 | 0.102 ± 0.094 | 0.22 ± 0.12 |
| Scl/p210;Bmi1 (B-ALL) | 14.4 ± 2.0 | 2.8 ± 0.4 | 1048.8 ± 110.5 | 12.2 ± 2.1 | 89 ± 11* | 0.029 ± 0.009 | 0.066 ± 0.046 | 0.108 ± 0.073 | 16.5 ± 8.0* |
| Scl/p210;Bmi1 (non-B-ALL) | 11.0 ± 1.3 | 2.6 ± 1.6 | 808.0 ± 203.6 | 13.1 ± 1.9 | 41 ± 5 | 0.028 ± 0.002 | 0.084 ± 0.109 | 0.090 ± 0.014 | 0.362 ± 0.09 |
| Primary recipient group . | WBCs, × 103/mm3 . | ANC, × 103/mm3 . | PLTs, × 103/mm3 . | Hb, g/dL . | Spleen weight, mg . | EGFP+ LT-HSCs, % . | EGFP+ ST-HSCs, % . | EGFP+ MPPs, % . | EGFP+ B220lo, % . |
|---|---|---|---|---|---|---|---|---|---|
| Ntg;MOCK | 10.6 ± 1.9 | 2.7 ± 0.6 | 940.3 ± 101.3 | 14.4 ± 0.7 | 40 ± 10 | 0.031 ± 0.003 | 0.099 ± 0.066 | 0.098 ± 0.063 | 0.34 ± 0.10 |
| Ntg;Bmi1 | 8.7 ± 2.3 | 2.5 ± 0.7 | 931.0 ± 181.4 | 13.0 ± 1.6 | 40 ± 5 | 0.021 ± 0.003 | 0.106 ± 0.070 | 0.118 ± 0.068 | 0.42 ± 0.05 |
| Scl/p210;MOCK | 12.6 ± 3.6 | 2.7 ± 0.7 | 999.8 ± 90.8 | 13.0 ± 0.6 | 49 ± 13 | 0.038 ± 0.005 | 0.106 ± 0.108 | 0.102 ± 0.094 | 0.22 ± 0.12 |
| Scl/p210;Bmi1 (B-ALL) | 14.4 ± 2.0 | 2.8 ± 0.4 | 1048.8 ± 110.5 | 12.2 ± 2.1 | 89 ± 11* | 0.029 ± 0.009 | 0.066 ± 0.046 | 0.108 ± 0.073 | 16.5 ± 8.0* |
| Scl/p210;Bmi1 (non-B-ALL) | 11.0 ± 1.3 | 2.6 ± 1.6 | 808.0 ± 203.6 | 13.1 ± 1.9 | 41 ± 5 | 0.028 ± 0.002 | 0.084 ± 0.109 | 0.090 ± 0.014 | 0.362 ± 0.09 |
P < .01 comparing Scl/p210;MOCK and Scl/p210;Bmi1 mice (n = 3-5 mice per group). Scl/p210;Bmi1 mice died with features of B-ALL (hind limb paralysis, lymphadenopathy, and infiltration of lymphoblasts in different tissues).
WBC indicates white blood cells; ANC, absolute neutrophil count; PLTs, platelets; Hb, hemoglobin; LT, long-term; HSC, hematotpoietic stem cells; and ST, short-term.