Chromosomal instability of bcasp8−/− lymphomas
| . | Cell 1 . | Cell 2 . | Cell 3 . | Cell 4 . | Cell 5 . | Range . | Average . |
|---|---|---|---|---|---|---|---|
| bcasp8−/− tumor no. 1 | |||||||
| No. of chromosomes | 84 | 75 | 82 | 83 | 83 | 75-84 | 81.4 |
| Dicentrics/tetracentrics | 14 | 9 | 19 | 17 | 15 | 9-19 | 14.8 |
| Acentrics/double minute | 8 | 12 | 12 | 17 | 9 | 8-17 | 11.6 |
| Chromosome with deletion | 16 | 5 | 10 | 14 | 15 | 5-16 | 12 |
| Chromosome with probable duplication | 2 | 1 | 0 | 1 | 1 | 0-2 | 1 |
| Tri-radials | 1 | 1 | 0 | 0 | 1 | 0-1 | 0.6 |
| Ring chromosomes | 0 | 0 | 0 | 3 | 1 | 0-3 | 0.8 |
| Abnormally fused chromosome segments | 42 | 25 | 45 | 40 | 32 | 25-45 | 36.8 |
| Total no. of changes per cell | 83 | 53 | 86 | 92 | 74 | 53-92 | 77.6 |
| Total no. of changes per chromosome | 0.99 | 0.71 | 1.05 | 1.11 | 0.89 | 0.95 | |
| bcasp8−/− tumor no. 2 | |||||||
| No. of chromosomes | 81 | 79 | 80 | 87 | 67 | 67-81 | 78.8 |
| Dicentrics/tetracentrics | 11 | 18 | 15 | 14 | 10 | 10-18 | 13.6 |
| Acentrics/double minute | 7 | 11 | 17 | 16 | 10 | 7-16 | 12.2 |
| Chromosome with deletion | 14 | 8 | 7 | 10 | 8 | 7-14 | 9.4 |
| Chromosome with probable duplication | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Tri-radials | 0 | 0 | 1 | 0 | 2 | 0-2 | 0.6 |
| Ring chromosomes | 0 | 0 | 0 | 1 | 1 | 0-1 | 0.5 |
| Abnormally fused chromosome segments | 26 | 26 | 20 | 36 | 18 | 20-36 | 25.2 |
| Total no. of changes per cell | 58 | 63 | 60 | 77 | 49 | 49-77 | 61.4 |
| Total no. of changes per chromosome | 0.72 | 0.8 | 0.75 | 0.89 | 0.73 | 0.78 |
| . | Cell 1 . | Cell 2 . | Cell 3 . | Cell 4 . | Cell 5 . | Range . | Average . |
|---|---|---|---|---|---|---|---|
| bcasp8−/− tumor no. 1 | |||||||
| No. of chromosomes | 84 | 75 | 82 | 83 | 83 | 75-84 | 81.4 |
| Dicentrics/tetracentrics | 14 | 9 | 19 | 17 | 15 | 9-19 | 14.8 |
| Acentrics/double minute | 8 | 12 | 12 | 17 | 9 | 8-17 | 11.6 |
| Chromosome with deletion | 16 | 5 | 10 | 14 | 15 | 5-16 | 12 |
| Chromosome with probable duplication | 2 | 1 | 0 | 1 | 1 | 0-2 | 1 |
| Tri-radials | 1 | 1 | 0 | 0 | 1 | 0-1 | 0.6 |
| Ring chromosomes | 0 | 0 | 0 | 3 | 1 | 0-3 | 0.8 |
| Abnormally fused chromosome segments | 42 | 25 | 45 | 40 | 32 | 25-45 | 36.8 |
| Total no. of changes per cell | 83 | 53 | 86 | 92 | 74 | 53-92 | 77.6 |
| Total no. of changes per chromosome | 0.99 | 0.71 | 1.05 | 1.11 | 0.89 | 0.95 | |
| bcasp8−/− tumor no. 2 | |||||||
| No. of chromosomes | 81 | 79 | 80 | 87 | 67 | 67-81 | 78.8 |
| Dicentrics/tetracentrics | 11 | 18 | 15 | 14 | 10 | 10-18 | 13.6 |
| Acentrics/double minute | 7 | 11 | 17 | 16 | 10 | 7-16 | 12.2 |
| Chromosome with deletion | 14 | 8 | 7 | 10 | 8 | 7-14 | 9.4 |
| Chromosome with probable duplication | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Tri-radials | 0 | 0 | 1 | 0 | 2 | 0-2 | 0.6 |
| Ring chromosomes | 0 | 0 | 0 | 1 | 1 | 0-1 | 0.5 |
| Abnormally fused chromosome segments | 26 | 26 | 20 | 36 | 18 | 20-36 | 25.2 |
| Total no. of changes per cell | 58 | 63 | 60 | 77 | 49 | 49-77 | 61.4 |
| Total no. of changes per chromosome | 0.72 | 0.8 | 0.75 | 0.89 | 0.73 | 0.78 |
Chromosomal abnormalities are described as recommended by International Standing Committee on Human Cytogenetic Nomenclature22 and following the recommendations of Savage et al.23 The abnormally fused chromosomal segments are identified as per a SKY color variation on a derivative chromosome (eg, a derivative chromosome composed of 3 different chromosome segments would have a score of 2).