Half-maximum (IC50) and maximum inhibitory (Imax) concentration values for the inhibition of endothelial cells proliferation by CXCL4, CXCL4L1, and related mutants
| . | IC50, mg/mL . | Imax, mg/mL . |
|---|---|---|
| rCXCL4 | 2.3 | 5.0 |
| rCXCL4L1 (v1) | 5.3 × 10−2 | 0.2 |
| rCXCL4 P58L(L) | 2.0 | 5.0 |
| rCXCL4 K66E (E) | 2.0 | 5.0 |
| rCXCL4 P58L K66E (LE) | 0.85 | 2.5 |
| rCXCL4 P58L L67H (LH) | 4.2 × 10−2 | 0.2 |
| rCXCL4 K66E L67H (EH) | 8.9 × 10−2 | 0.25 |
| rCXCL4 241 (241) | 0.84 | 5.0 |
| rCXCL4 241 L67H (241+H) | 15 × 10−2 | 0.4 |
| . | IC50, mg/mL . | Imax, mg/mL . |
|---|---|---|
| rCXCL4 | 2.3 | 5.0 |
| rCXCL4L1 (v1) | 5.3 × 10−2 | 0.2 |
| rCXCL4 P58L(L) | 2.0 | 5.0 |
| rCXCL4 K66E (E) | 2.0 | 5.0 |
| rCXCL4 P58L K66E (LE) | 0.85 | 2.5 |
| rCXCL4 P58L L67H (LH) | 4.2 × 10−2 | 0.2 |
| rCXCL4 K66E L67H (EH) | 8.9 × 10−2 | 0.25 |
| rCXCL4 241 (241) | 0.84 | 5.0 |
| rCXCL4 241 L67H (241+H) | 15 × 10−2 | 0.4 |
The half maximum (IC50) and maximum inhibitory (Imax) concentration for each protein was deduced from the dose-response curves (supplemental Figure 3) of the inhibition of endothelial cell proliferation.