Bacterial strains used in this study
| Strain . | Characteristic . | Source or reference . |
|---|---|---|
| S enterica sv Typhimurium wild-type strain | ||
| SL3770 | waa+, smooth LPS (S-form) | SGSC#225 |
| S enterica sv Typhimurium mutant strains | ||
| SL3749 | waaL446, lacking LPS O-antigen ligase | SGSC#228 |
| SL3750 | waaJ417, lacking LPS glucosyltransferase II | SGSC#229 |
| SL3748 | waaI432, lacking LPS galactosyltransferase I | SGSC#227 |
| SL3769 | waaG471, lacking LPS glucosyltransferase I | SGSC#231 |
| SL3789 | waaF511, lacking LPS heptosyltransferase II | SGSC#230 |
| SL1102 | hldE543, lacking LPS heptosyltransferase I | SGSC#258 |
| E coli K-12 wild-type strain | ||
| BW30270 | waa+, rough LPS (R-form) | CGSC#7925 |
| E coli K-12 mutant strains | ||
| KPM53 | waaC, lacking LPS heptosyltransferase I | Mamat et al18 |
| KPM121 | waaA, defective in Kdo transferase of LPS biosynthesis | Mamat et al17 |
| Strain . | Characteristic . | Source or reference . |
|---|---|---|
| S enterica sv Typhimurium wild-type strain | ||
| SL3770 | waa+, smooth LPS (S-form) | SGSC#225 |
| S enterica sv Typhimurium mutant strains | ||
| SL3749 | waaL446, lacking LPS O-antigen ligase | SGSC#228 |
| SL3750 | waaJ417, lacking LPS glucosyltransferase II | SGSC#229 |
| SL3748 | waaI432, lacking LPS galactosyltransferase I | SGSC#227 |
| SL3769 | waaG471, lacking LPS glucosyltransferase I | SGSC#231 |
| SL3789 | waaF511, lacking LPS heptosyltransferase II | SGSC#230 |
| SL1102 | hldE543, lacking LPS heptosyltransferase I | SGSC#258 |
| E coli K-12 wild-type strain | ||
| BW30270 | waa+, rough LPS (R-form) | CGSC#7925 |
| E coli K-12 mutant strains | ||
| KPM53 | waaC, lacking LPS heptosyltransferase I | Mamat et al18 |
| KPM121 | waaA, defective in Kdo transferase of LPS biosynthesis | Mamat et al17 |
SGSC indicates Salmonella Genetic Stock Center; and CGSC, E coli Genetic Stock Center.