Table 3 ISG subsets induced by HIV-1 in... | American Society of Hematology

Table 3

ISG subsets induced by HIV-1 in MDMs, MDDCs, and CD4 T cells compared with mock infection as quantified by quantitative PCR

GenBank accession no.	Gene name	MDMs				MDDCs			CD4 T cells
GenBank accession no.	Gene name	6 h	24 h	48 h	96 h	6 h	24 h	48 h	6 h	24 h	48 h	96 h
NM_080657	Viperin	33.9	30.9	16	1142.3	1.8	2.6	4.7	9.4	12.9	10	14
NM_001548	IFIT1	8.4	18.3	9.2	282.2	4.4	6	3.8	199	79	51	58
NM_003641	IFITM1	3.1	6.5	6.7	221.5	2.2	5.7	15.2	8.9	4.2	6.8	NC
NM_001549	IFIT3	NC	11.2	6.2	151.7	3.8	9.7	18	26	20.6	13.2	36.1
NM_001547	IFIT2	10	21.6	19.3	144.4	4.5	6	5	8.3	22.5	8.9	42.9
NM_002462	MXI	6.5	4.1	2.0	72.2	3.2	7	7.8	4.5	12.6	4.3	21.6
NM_006820	IFI44L	2.3	4.9	16	60.1	ND	ND	ND	9.9	23.9	19.5	12.6
NM_014314	RIGI	4.9	3.1	5	56.9	NC	6.2	18.4	14.6	1.8	2.5	3
NM_005101	ISG15	1.6	6	5.1	52.2	1.8	9.5	8	17.2	13.4	16.7	31
NM_006187	OAS3	NC	3.8	NC	42.0	2.3	4.8	5.5	4.4	5.1	5.4	4.5
NM_022168	MDA5	7.7	14.3	1.3	40.2	NC	8.3	12.9	5.7	3.2	5.6	2.8
NM_016817	OAS2	2.1	1.7	3.5	33.7	ND	ND	ND	6.4	23.4	4.8	6.7
NM_021034	IFITM3	NC	3.5	1.6	26.4	2.3	4.8	4.4	2.5	18.1	2.5	3
NM_005533	IFI35	NC	8.8	2.6	17.7	1.8	2.1	5.1	NC	4.4	3.8	10.9
NM_016816	OAS1	1.7	2.8	NC	16.4	2	5.5	3	2.9	12.4	4.45	51.9
NM_002759	PKR	NC	2.4	3	15.5	1.6	3.1	2.5	4.1	3.7	3.8	6.6
NM_012420	IFIT5	2	13.7	NC	3.9	NC	2	3.8	5.6	4	2.6	38.1
NM_006435	IFITM2	NC	NC	NC	3.2	ND	ND	ND	6.7	6.6	7.4	4.7
NM_135276	STAT3	NC	NC	NC	3.1	NC	3.5	2.9	NC	NC	NC	NC
NM_004031	IRF7	1.9	5.4	3.1	2.8	3.9	5	4.9	2.1	8.1	7.2	1.8
NM_001571	IRF3	NC	NC	2.4	2.3	NC	NC	NC	NC	NC	NC	NC
NM_002198	IRF1	5	10.2	2.3	1.7	2.7	4.6	7.4	2.7	1.9	5.1	0.6
NM_006084	IRF9	ND	NC	NC	1.6	NC	3.8	1.8	NC	2.4	4.6	2.8
NM_003955	SOCS3	NC	NC	3.1	1.5	1.8	2	8	2.6	1.5	3.8	1.8
NM_002199	IRF2		5	NC	NC	NC	1.7	2.7	NC	NC	1.7	0.8
NM_000628	IL10RB		1.6	NC	NC	NC	3.9	2.6	1.9	NC	6	3.1
NM_002460	IRF4	ND	2	6.7	ND	NC	4	4.4	6.8	15.1	11.5	5.4
NM_002163	IRF8	NC	7.2	NC	NC	8.9	2.7	NC	2.3	NC	1.8	NC
All isoforms	IFNα	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC
NM_002176	IFNβ	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC
NM_000619	IFNγ	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC
NM_002177	IFNω	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND
NM_172138	IL-28a	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND
NM_172139	IL-28b	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND
NM_172140	IL-29	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND

GenBank accession no.	Gene name	MDMs				MDDCs			CD4 T cells
GenBank accession no.	Gene name	6 h	24 h	48 h	96 h	6 h	24 h	48 h	6 h	24 h	48 h	96 h
NM_080657	Viperin	33.9	30.9	16	1142.3	1.8	2.6	4.7	9.4	12.9	10	14
NM_001548	IFIT1	8.4	18.3	9.2	282.2	4.4	6	3.8	199	79	51	58
NM_003641	IFITM1	3.1	6.5	6.7	221.5	2.2	5.7	15.2	8.9	4.2	6.8	NC
NM_001549	IFIT3	NC	11.2	6.2	151.7	3.8	9.7	18	26	20.6	13.2	36.1
NM_001547	IFIT2	10	21.6	19.3	144.4	4.5	6	5	8.3	22.5	8.9	42.9
NM_002462	MXI	6.5	4.1	2.0	72.2	3.2	7	7.8	4.5	12.6	4.3	21.6
NM_006820	IFI44L	2.3	4.9	16	60.1	ND	ND	ND	9.9	23.9	19.5	12.6
NM_014314	RIGI	4.9	3.1	5	56.9	NC	6.2	18.4	14.6	1.8	2.5	3
NM_005101	ISG15	1.6	6	5.1	52.2	1.8	9.5	8	17.2	13.4	16.7	31
NM_006187	OAS3	NC	3.8	NC	42.0	2.3	4.8	5.5	4.4	5.1	5.4	4.5
NM_022168	MDA5	7.7	14.3	1.3	40.2	NC	8.3	12.9	5.7	3.2	5.6	2.8
NM_016817	OAS2	2.1	1.7	3.5	33.7	ND	ND	ND	6.4	23.4	4.8	6.7
NM_021034	IFITM3	NC	3.5	1.6	26.4	2.3	4.8	4.4	2.5	18.1	2.5	3
NM_005533	IFI35	NC	8.8	2.6	17.7	1.8	2.1	5.1	NC	4.4	3.8	10.9
NM_016816	OAS1	1.7	2.8	NC	16.4	2	5.5	3	2.9	12.4	4.45	51.9
NM_002759	PKR	NC	2.4	3	15.5	1.6	3.1	2.5	4.1	3.7	3.8	6.6
NM_012420	IFIT5	2	13.7	NC	3.9	NC	2	3.8	5.6	4	2.6	38.1
NM_006435	IFITM2	NC	NC	NC	3.2	ND	ND	ND	6.7	6.6	7.4	4.7
NM_135276	STAT3	NC	NC	NC	3.1	NC	3.5	2.9	NC	NC	NC	NC
NM_004031	IRF7	1.9	5.4	3.1	2.8	3.9	5	4.9	2.1	8.1	7.2	1.8
NM_001571	IRF3	NC	NC	2.4	2.3	NC	NC	NC	NC	NC	NC	NC
NM_002198	IRF1	5	10.2	2.3	1.7	2.7	4.6	7.4	2.7	1.9	5.1	0.6
NM_006084	IRF9	ND	NC	NC	1.6	NC	3.8	1.8	NC	2.4	4.6	2.8
NM_003955	SOCS3	NC	NC	3.1	1.5	1.8	2	8	2.6	1.5	3.8	1.8
NM_002199	IRF2		5	NC	NC	NC	1.7	2.7	NC	NC	1.7	0.8
NM_000628	IL10RB		1.6	NC	NC	NC	3.9	2.6	1.9	NC	6	3.1
NM_002460	IRF4	ND	2	6.7	ND	NC	4	4.4	6.8	15.1	11.5	5.4
NM_002163	IRF8	NC	7.2	NC	NC	8.9	2.7	NC	2.3	NC	1.8	NC
All isoforms	IFNα	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC
NM_002176	IFNβ	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC
NM_000619	IFNγ	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC	NC
NM_002177	IFNω	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND
NM_172138	IL-28a	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND
NM_172139	IL-28b	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND
NM_172140	IL-29	NC	NC	NC	NC	ND	ND	ND	ND	ND	ND	ND

MDMs, MDDCs, and CD4 T cells were mock or HIV-1 infected. RNA was extracted at 6, 24, 48, and 96 hours. It was reverse transcribed, and gene expression was determined by quantitative PCR relative to GAPDH. Data represent mean fold change of 3 donors.

NC indicates no change in expression between mock and infected; and ND, not done.

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