Defective immunosuppression by AA Tregs contributed to impaired hematopoiesis
| . | CFU per 250 cord blood CD34+ cells, mean ± SEM, n = 6 . | |||
|---|---|---|---|---|
| Tresp . | Tresp and Tregs . | Tcyt . | Tcyt and Tregs . | |
| CFU-total | ||||
| Controls | 79.8 ± 12.6 | 160.3 ± 14.1 | 71.0 ± 10.4 | 139.3 ± 15.8 |
| AA | 57.3 ± 2.8 | 80.3 ± 7.1 | 48.8 ± 5.0 | 72.0 ± 8.2 |
| P | .002 | < .001 | .001 | < .001 |
| CFU-GM | ||||
| Controls | 54.1 ± 8.8 | 110.5 ± 16.6 | 49.8 ± 7.6 | 98.2 ± 13.3 |
| AA | 43.0 ± 4.9 | 58.7 ± 6.8 | 35.5 ± 4.8 | 52.2 ± 7.1 |
| P | .022 | < .001 | .003 | < .001 |
| BFU-E | ||||
| Controls | 25.5 ± 6.2 | 47.5 ± 9.5 | 20.7 ± 4.5 | 38.8 ± 7.8 |
| AA | 14.2 ± 3.3 | 20.3 ± 7.0 | 12.8 ± 3.2 | 19.0 ± 6.2 |
| P | .04 | < .001 | .006 | .001 |
| CFU-Mix | ||||
| Controls | 1.0 ± 0.6 | 2.3 ± 0.8 | 0.5 ± 0.5 | 2.3 ± 0.5 |
| AA | 0.2 ± 0.4 | 0.7 ± 0.8 | 0.3 ± 0.5 | 0.8 ± 0.8 |
| P | .022 | .005 | .599 | .002 |
| . | CFU per 250 cord blood CD34+ cells, mean ± SEM, n = 6 . | |||
|---|---|---|---|---|
| Tresp . | Tresp and Tregs . | Tcyt . | Tcyt and Tregs . | |
| CFU-total | ||||
| Controls | 79.8 ± 12.6 | 160.3 ± 14.1 | 71.0 ± 10.4 | 139.3 ± 15.8 |
| AA | 57.3 ± 2.8 | 80.3 ± 7.1 | 48.8 ± 5.0 | 72.0 ± 8.2 |
| P | .002 | < .001 | .001 | < .001 |
| CFU-GM | ||||
| Controls | 54.1 ± 8.8 | 110.5 ± 16.6 | 49.8 ± 7.6 | 98.2 ± 13.3 |
| AA | 43.0 ± 4.9 | 58.7 ± 6.8 | 35.5 ± 4.8 | 52.2 ± 7.1 |
| P | .022 | < .001 | .003 | < .001 |
| BFU-E | ||||
| Controls | 25.5 ± 6.2 | 47.5 ± 9.5 | 20.7 ± 4.5 | 38.8 ± 7.8 |
| AA | 14.2 ± 3.3 | 20.3 ± 7.0 | 12.8 ± 3.2 | 19.0 ± 6.2 |
| P | .04 | < .001 | .006 | .001 |
| CFU-Mix | ||||
| Controls | 1.0 ± 0.6 | 2.3 ± 0.8 | 0.5 ± 0.5 | 2.3 ± 0.5 |
| AA | 0.2 ± 0.4 | 0.7 ± 0.8 | 0.3 ± 0.5 | 0.8 ± 0.8 |
| P | .022 | .005 | .599 | .002 |
Correlation analyses were carried out using the Student t test.
AA indicates aplastic anemia; Treg, regulatory T cell; Tresp, responder T cell; Tcyt, cytotoxic T cell; CFU-GM, CFU–granulocyte, macrophage; BFU-E, burst-forming unit–erythroid; and CFU-Mix, CFU–granulocyte, erythroid, macrophage, megakaryocyte.