Percentage of PA Ab binding to human-mouse chimeric αIIbs complexed with human β3 relative to PA Ab binding to wt αIIbβ3 in 293T cells
| . | m(W1:4-1)H . | m(W1:1-2)H . | m(W1:2-3)H . | m(W2:2-3)H . | m(W2:3-4)H . | m(W3:4-1)H . | m(W4:4-1)H . |
|---|---|---|---|---|---|---|---|
| Group A | |||||||
| PT 17 | 99.5* | −1.5* | −1.7 | −4.7 | NT | −2.9 | 0.3 |
| PT 23 | 72.7* | 18.9* | −25.0 | −18.4 | −6.6 | −20.6 | 15.2 |
| Group B | |||||||
| PT 3 | 84.4 | 83.3* | 19.6* | 12.7 | NT | 2.9 | 7.9 |
| PT 36 | 134.2 | 116.6* | 1.5* | NT | 1.7 | 2.6 | 3.8 |
| PT 41 | 88.7 | 97.5* | 50.3* | NT | 51.0 | 44.6 | 22.3 |
| PT 42 | 85.6 | 105.0* | 47.5* | NT | 41.3 | 20.5 | 13.5 |
| PT 12 | 89.7 | 87.1 | 57.5 | 30.9 | 9.1 | 6.8 | −1.7 |
| Group C | |||||||
| PT 1 | 75.2 | NT | 93.2 | 93.7 | 102.2* | 27.3* | 13.0 |
| PT 6 | 109.1 | NT | 232.2 | 115.9 | 103.1* | 30.7* | 30.7 |
| PT 34 | 94.0 | 91.7 | 108.5 | NT | 88.3* | −3.4* | 12.1 |
| PT 45 | 94.3 | 91.4 | 95.3 | NT | 73.5* | 13.5* | 6.5 |
| Others | |||||||
| PT 2 | 87.5 | 80.3 | 45.8 | 36.0 | NT | 19.6 | 18.9 |
| PT 5 | 101.5 | 82.4 | 74.0 | 61.6 | 43.4 | 39.7 | 18.3 |
| PT 7 | 104.9 | NT | 111.4 | 90.5 | 105.3 | 60.3 | 48.6 |
| PT 37 | 123.5 | 128.5 | 102.8 | NT | 114.1 | 65.4 | 45.9 |
| . | m(W1:4-1)H . | m(W1:1-2)H . | m(W1:2-3)H . | m(W2:2-3)H . | m(W2:3-4)H . | m(W3:4-1)H . | m(W4:4-1)H . |
|---|---|---|---|---|---|---|---|
| Group A | |||||||
| PT 17 | 99.5* | −1.5* | −1.7 | −4.7 | NT | −2.9 | 0.3 |
| PT 23 | 72.7* | 18.9* | −25.0 | −18.4 | −6.6 | −20.6 | 15.2 |
| Group B | |||||||
| PT 3 | 84.4 | 83.3* | 19.6* | 12.7 | NT | 2.9 | 7.9 |
| PT 36 | 134.2 | 116.6* | 1.5* | NT | 1.7 | 2.6 | 3.8 |
| PT 41 | 88.7 | 97.5* | 50.3* | NT | 51.0 | 44.6 | 22.3 |
| PT 42 | 85.6 | 105.0* | 47.5* | NT | 41.3 | 20.5 | 13.5 |
| PT 12 | 89.7 | 87.1 | 57.5 | 30.9 | 9.1 | 6.8 | −1.7 |
| Group C | |||||||
| PT 1 | 75.2 | NT | 93.2 | 93.7 | 102.2* | 27.3* | 13.0 |
| PT 6 | 109.1 | NT | 232.2 | 115.9 | 103.1* | 30.7* | 30.7 |
| PT 34 | 94.0 | 91.7 | 108.5 | NT | 88.3* | −3.4* | 12.1 |
| PT 45 | 94.3 | 91.4 | 95.3 | NT | 73.5* | 13.5* | 6.5 |
| Others | |||||||
| PT 2 | 87.5 | 80.3 | 45.8 | 36.0 | NT | 19.6 | 18.9 |
| PT 5 | 101.5 | 82.4 | 74.0 | 61.6 | 43.4 | 39.7 | 18.3 |
| PT 7 | 104.9 | NT | 111.4 | 90.5 | 105.3 | 60.3 | 48.6 |
| PT 37 | 123.5 | 128.5 | 102.8 | NT | 114.1 | 65.4 | 45.9 |
The PT no. designates the ITP sample that was tested for binding to cells that expressed the indicated chimeras. Values are percentages and represent the means of ≥ 2 independent experiments.
NT indicates not tested.
Marked reductions (> 40%) in binding between 2 loops in addition to marked reduction (> 40%) in binding compared with binding with wt αIIbβ3.