Allosteric activation of plasma ADAMTS13 for selected vertebrates
| Plasma species . | VWF71 cleavage normalized %* . | Activation (fold) by† . | ||
|---|---|---|---|---|
| pH 6/pH 7.4 . | mAbs . | VWF D4 . | ||
| Goat | 1.2 ± 0.2* | <LoD | 2.6 ± 0.1 | 1.84 ± 0.05 |
| Rat | 1.6 ± 0.4 | 1.7 ± 0.1 | 1.2 ± 0.2 | 1.8 ± 0.1 |
| Cattle | 1.7 ± 0.7 | 2.4 ± 0.4 | 5.3 ± 0.4 | 2.2 ± 0.6 |
| Sheep | 1.9 ± 0.9 | 0.4 ± 0.1 | 3.4 ± 0.8 | 2.4 ± 0.1 |
| Chicken | 2.3 ± 1.0 | <LoD | 1.04 ± 0.02 | 1.47 ± 0.23 |
| Horse | 8.0 ± 2.8 | 1.6 ± 0.1 | 26 ± 12† | 2.73 ± 0.01 |
| FVB mouse | 12.6 ± 1.22 | 2.1 ± 0.2 | 1.8 ± 0.2 | 1.1 ± 0.1 |
| Marmoset | 20 ± 8 | 1.6 ± 0.4 | 3.4 ± 0.2 | 1.2 ± 0.1 |
| Pig | 21 ± 6 | 2.3 ± 0.1 | 6.3 ± 0.3 | 3.7 ± 0.1 |
| Pigeon | 26 ± 4 | 15.7 ± 0.6 | 1.02 ± 0.02 | 6.4 ± 0.3 |
| C57BL/6 mouse | 26 ± 2 | 1.11 ± 0.01 | 1.01 ± 0.01 | 0.97 ± 0.01 |
| Frog | 26 ± 26 | 4.6 ± 0.3 | 1.00 ± 0.001 | 5.6 ± 0.2 |
| Baboon | 71 ± 8.03 | 2.5 ± 0.3 | 4.5 ± 0.6 | 1.9 ± 0.1 |
| Chimpanzee | 82 ± 6* | 2.08 ± 0.01 | 7.4 ± 0.7 | 2.02 ± 0.02 |
| Macaque | 86 ± 34 | 2.9 ± 0.2 | 13 ± 1 | 2.9 ± 0.0 |
| Ferret | 90 ± 2* | 1.26 ± 0.01 | 1.03 ± 0.06 | 1.52 ± 0.01 |
| Human | (100) | 3.1 ± 0.2 | 4.24 ± 0.04 | 1.83 ± 0.05 |
| Polar bear | 204 ± 7* | 1.9 ± 0.1 | 1.14 ± 0.02 | 1.32 ± 0.09 |
| Armadillo | 240 ± 100 | 0.01 ± 0.01 | 0.99 ± 0.02 | 0.85 ± 0.04 |
| Rabbit | 290 ± 60 | 1.15 ± 0.12 | 1.03 ± 0.02 | 3.6 ± 0.5 |
| Dog | 400 ± 10 | 0.19 ± 0.02 | 1.15 ± 0.05 | 1.1 ± 0.1 |
| Plasma species . | VWF71 cleavage normalized %* . | Activation (fold) by† . | ||
|---|---|---|---|---|
| pH 6/pH 7.4 . | mAbs . | VWF D4 . | ||
| Goat | 1.2 ± 0.2* | <LoD | 2.6 ± 0.1 | 1.84 ± 0.05 |
| Rat | 1.6 ± 0.4 | 1.7 ± 0.1 | 1.2 ± 0.2 | 1.8 ± 0.1 |
| Cattle | 1.7 ± 0.7 | 2.4 ± 0.4 | 5.3 ± 0.4 | 2.2 ± 0.6 |
| Sheep | 1.9 ± 0.9 | 0.4 ± 0.1 | 3.4 ± 0.8 | 2.4 ± 0.1 |
| Chicken | 2.3 ± 1.0 | <LoD | 1.04 ± 0.02 | 1.47 ± 0.23 |
| Horse | 8.0 ± 2.8 | 1.6 ± 0.1 | 26 ± 12† | 2.73 ± 0.01 |
| FVB mouse | 12.6 ± 1.22 | 2.1 ± 0.2 | 1.8 ± 0.2 | 1.1 ± 0.1 |
| Marmoset | 20 ± 8 | 1.6 ± 0.4 | 3.4 ± 0.2 | 1.2 ± 0.1 |
| Pig | 21 ± 6 | 2.3 ± 0.1 | 6.3 ± 0.3 | 3.7 ± 0.1 |
| Pigeon | 26 ± 4 | 15.7 ± 0.6 | 1.02 ± 0.02 | 6.4 ± 0.3 |
| C57BL/6 mouse | 26 ± 2 | 1.11 ± 0.01 | 1.01 ± 0.01 | 0.97 ± 0.01 |
| Frog | 26 ± 26 | 4.6 ± 0.3 | 1.00 ± 0.001 | 5.6 ± 0.2 |
| Baboon | 71 ± 8.03 | 2.5 ± 0.3 | 4.5 ± 0.6 | 1.9 ± 0.1 |
| Chimpanzee | 82 ± 6* | 2.08 ± 0.01 | 7.4 ± 0.7 | 2.02 ± 0.02 |
| Macaque | 86 ± 34 | 2.9 ± 0.2 | 13 ± 1 | 2.9 ± 0.0 |
| Ferret | 90 ± 2* | 1.26 ± 0.01 | 1.03 ± 0.06 | 1.52 ± 0.01 |
| Human | (100) | 3.1 ± 0.2 | 4.24 ± 0.04 | 1.83 ± 0.05 |
| Polar bear | 204 ± 7* | 1.9 ± 0.1 | 1.14 ± 0.02 | 1.32 ± 0.09 |
| Armadillo | 240 ± 100 | 0.01 ± 0.01 | 0.99 ± 0.02 | 0.85 ± 0.04 |
| Rabbit | 290 ± 60 | 1.15 ± 0.12 | 1.03 ± 0.02 | 3.6 ± 0.5 |
| Dog | 400 ± 10 | 0.19 ± 0.02 | 1.15 ± 0.05 | 1.1 ± 0.1 |
The concentration of VWF D4 was 20 μM.
FVB, Friend virus B; <LoD, below limit of detection; mAbs, mixture of 7C4, 19H4, and 12D4.
Entries represent the mean ± standard deviation normalized to human plasma for at least 3 individuals of each species except chimpanzee, ferret, goat, and polar bear, which are mean ± standard error for single individuals.
Values (±standard error) are based on at least 2 independent determinations, except horse shows mean ± standard deviation for 9 animals.