CALR del52 increases the thermal stability of TpoR
| Constructs expressed . | Apparent Tm ± SD of corresponding TpoR/EpoR construct (°C) . | P . |
|---|---|---|
| TpoR WT | 48.35 | |
| TpoR WT/CALR WT | 48.26 ± 0.36 | <.0001 |
| TpoR WT/CALR del52 | 55.07 ± 0.05 | |
| TpoR WT/CALR ins5 | 49.10 ± 0.54 | |
| TpoR R102P/CALR WT | 46.75 ± 0.21 | <.0001 |
| TpoR R102P/CALR del52 | 50.67 ± 0.38 | |
| TpoR NtoQ/CALR WT | 48.31 ± 0.24 | |
| TpoR NtoQ/CALR del52 | 48.87 ± 0.016 | |
| TpoR 8A/CALR WT | 44.75 ± 0.18 | =.0154 |
| TpoR 8A/CALR del52 | 43.91 ± 0.31 | |
| EpoR WT/CALR WT | 49.10 ± 0.17 | |
| EpoR WT/CALR del52 | 49.40 ± 0.25 | |
| EpoR LFFPL/CALR WT | 47.37 ± 0.16 | =.0017 |
| EpoR LFFPL/CALR del52 | 49.19 ± 0.39 | |
| TpoR Cys less/CALR WT | 44.89 ± 0.004 | =.0002 |
| TpoR Cys less/CALR del52 | 48.62 ± 0.48 | |
| TpoR P106L/CALR WT | 43.07 ± 0.07 | <.0001 |
| TpoR P106L/CALR del52 | 46.71 ± 0.21 | |
| TpoR WT control littermate platelets | 43.64 ± 0.05 | <.0001 |
| TpoR WT/Calr del52 KI heterozygous platelet | 52.04 ± 0.28 |
| Constructs expressed . | Apparent Tm ± SD of corresponding TpoR/EpoR construct (°C) . | P . |
|---|---|---|
| TpoR WT | 48.35 | |
| TpoR WT/CALR WT | 48.26 ± 0.36 | <.0001 |
| TpoR WT/CALR del52 | 55.07 ± 0.05 | |
| TpoR WT/CALR ins5 | 49.10 ± 0.54 | |
| TpoR R102P/CALR WT | 46.75 ± 0.21 | <.0001 |
| TpoR R102P/CALR del52 | 50.67 ± 0.38 | |
| TpoR NtoQ/CALR WT | 48.31 ± 0.24 | |
| TpoR NtoQ/CALR del52 | 48.87 ± 0.016 | |
| TpoR 8A/CALR WT | 44.75 ± 0.18 | =.0154 |
| TpoR 8A/CALR del52 | 43.91 ± 0.31 | |
| EpoR WT/CALR WT | 49.10 ± 0.17 | |
| EpoR WT/CALR del52 | 49.40 ± 0.25 | |
| EpoR LFFPL/CALR WT | 47.37 ± 0.16 | =.0017 |
| EpoR LFFPL/CALR del52 | 49.19 ± 0.39 | |
| TpoR Cys less/CALR WT | 44.89 ± 0.004 | =.0002 |
| TpoR Cys less/CALR del52 | 48.62 ± 0.48 | |
| TpoR P106L/CALR WT | 43.07 ± 0.07 | <.0001 |
| TpoR P106L/CALR del52 | 46.71 ± 0.21 | |
| TpoR WT control littermate platelets | 43.64 ± 0.05 | <.0001 |
| TpoR WT/Calr del52 KI heterozygous platelet | 52.04 ± 0.28 |
Ba/F3 cells stably expressing TpoR/EpoR constructs along with CALR WT or CALR del52 or platelets from mice (KI-Calr del52/WT or control littermates) were used to analyze the thermal stability of the cytokine receptors. The thermal stability curves (supplemental Figure 8) were thus obtained for each cell type. The temperature at which 50% of the receptors were denatured was denoted as Tm. Table depicts the Tm ± standard deviation (SD) of at least 3 independent experiments. The unpaired Student t test was used to calculate the P values between the groups (indicated in the far-right column).