Clinical information, percentages of CLN, cytogenetic/FISH/microarray results, and day-29 MRD data for precursor B lymphoblastic leukemia cases with CLN
| Case . | Age, y . | Sex . | Average CLN, % . | Karyotype . | FISH for IKZF1 loss . | Array for IKZF1 loss . | Day-29 MRD, % . |
|---|---|---|---|---|---|---|---|
| 1 | 7 | F | 16 | 46,XX[20] | Positive | Entire gene | 0.05 |
| 2 | 7 | M | 14.8 | 47,XY,del(7)(p11.2),+21[9]/47,sl,add(9)(p21)[5]/46,XY[6]* | Positive | — | 0.38 |
| 3 | 14 | F | 13.8 | 46,XX,t(2;14)(p11.2;q11.2),del(9)(p21)[5]/46,XX[15]* | Positive | — | 0 |
| 4 | 5 | F | 12.7 | 46,XX[20] | Positive | Entire gene | 0 |
| 5 | 0.7 | M | 11.5 | 46,XY,t(4;11)(q21;q23)[15]/46,XY[5] | Positive | — | 0 |
| 6 | 10 | F | 6.5 | 47,XX,+21c[20](DS) | — | — | 0.01 |
| 7 | 8 | F | 5.2 | 46,XX[20] | Negative | — | 0 |
| 8 | 7 | M | 5.1 | 47,XY,+21[4]/46,XY,add(6)(q21)[3]/ 46,XY[21] | Positive | — | 0 |
| 9 | 3 | M | 4.7 | 46,XY,add(14)(q32)[11] | Positive | — | — |
| 10 | 1.9 | M | 4.6 | 46,XY,t(1;11)(p32;q23)[14]/46,XY[6] | Positive | — | — |
| 11 | 3.8 | M | 4.3 | 46,XY[20] | Negative | — | 0 |
| 12 | 0.7 | F | 4.3 | — | Negative | — | — |
| 13 | 12 | F | 4.2 | 46,XX,add(5)(q31),t(6;13)(q21;q34)[11]/46,XX[9] | Positive | — | 0.36 |
| 14 | 3 | M | 4.1 | 45,XY,der(12)t(12;13)(p13;q12),-13[4]/46,XY[16]† | Positive | — | 0 |
| 15 | 4.7 | F | 3.9 | 49,XX,+17,+21,+mar[6]/46,XX[10] | Positive | — | 0 |
| 16 | 4.6 | M | 3.7 | 46,XY,add(3)(p14),add(8)(q22),add(12)(p11.1),der(12)t(3;12)(p14;p11.1),i(21)(q10)[17]/46,XY[3]† | Negative | — | 0 |
| 17 | 6 | M | 3.6 | 46,XY[20] | Positive | Exons 4-6 | 0.02 |
| 18 | 18 | F | 3.4 | 46,XY[20] | — | — | 2.7 |
| 19 | 3.8 | M | 3.3 | 46,XY[20] | Negative | — | 0 |
| 20 | 15 | M | 3.3 | 47,XY,+5[20] | Positive | Exons 4-6 | 1.1 |
| 21 | 12 | M | 3.2 | 46,XY[20] | — | — | 0.04 |
| 22 | 3.1 | F | 3 | 46,XX,i(7)(q10),+21c[10]/47,XX,+21c[10](DS) | — | — | 0 |
| 23 | 7 | F | 2.9 | 46,XX[20] | Negative | — | 0 |
| 24 | 16 | M | 2.7 | 46,XY,add(3)(p13),add(5)(q11.2),add(9)(p12),der(9)add(p11)add(q22)[14]/46,XY[4]* | Negative | — | 0 |
| 25 | 2.8 | F | 2.7 | 46,XX,add(12)(p13)[5]/46,XX[15]† | Positive | — | 0 |
| 26‡ | 7 | M | 2.6 | 46,XY,del(5)(q13),der(9)t(5;9)(9qter→9q33::9p21→9q33::5q13→5qter),del(9)(p21)[18]/46,XY[2]* | Positive | — | 0 |
| 27 | 4.1 | M | 2.5 | 45,XY,-8,der(12)t(8;12)(q11.2;p11.2)[10]/46,XY[10]† | Positive | — | 0 |
| 28 | 12 | F | 2.5 | 46,XX,add(1)(p21),add(7)(q11.2),-8,-13,-13, add(16)(q22),−21,+4mar[5]/46,XX[10]§ | Negative | — | 0 |
| 29 | 8 | F | 2.4 | 46,XX[20] | Positive | — | 0 |
| 30 | 1.8 | F | 2.4 | 47,XX,+5,r(9),add(13)(q14)[5]/47,sl,add(7)(q32)[12]/46,XX[3]* | Positive | — | 0 |
| 31 | 3.9 | M | 2.4 | 46,XY[20]¶ | Positive | — | 0 |
| 32|| | 4.4 | M | 2.3 | 46,XY,t(9;22)(q34;q11.2)[1]/46,idem,idic(Y)(p11.3),t(1;2)(p36.1;p12)[16]/46,XY[3]# | Positive | — | — |
| 33 | 3 | M | 2.2 | 46,XY,dic(9;20)(p13;q11.2),+21[20]/46,XY[1]* | Positive | — | 0.09 |
| 34 | 12 | M | 2.2 | 46,XX,r(7),+9[14]/46,XX[6] | Positive | — | 0 |
| 35 | 9 | M | 2.15 | — | — | — | 0 |
| 36 | 1.5 | M | 2 | 60,XY,+X,+4,+6,+8,+10,+11,+12,+14,+14,+17,+18,+21,+21,+22[9]/46,XY[11] | Positive | — | 0 |
| Case . | Age, y . | Sex . | Average CLN, % . | Karyotype . | FISH for IKZF1 loss . | Array for IKZF1 loss . | Day-29 MRD, % . |
|---|---|---|---|---|---|---|---|
| 1 | 7 | F | 16 | 46,XX[20] | Positive | Entire gene | 0.05 |
| 2 | 7 | M | 14.8 | 47,XY,del(7)(p11.2),+21[9]/47,sl,add(9)(p21)[5]/46,XY[6]* | Positive | — | 0.38 |
| 3 | 14 | F | 13.8 | 46,XX,t(2;14)(p11.2;q11.2),del(9)(p21)[5]/46,XX[15]* | Positive | — | 0 |
| 4 | 5 | F | 12.7 | 46,XX[20] | Positive | Entire gene | 0 |
| 5 | 0.7 | M | 11.5 | 46,XY,t(4;11)(q21;q23)[15]/46,XY[5] | Positive | — | 0 |
| 6 | 10 | F | 6.5 | 47,XX,+21c[20](DS) | — | — | 0.01 |
| 7 | 8 | F | 5.2 | 46,XX[20] | Negative | — | 0 |
| 8 | 7 | M | 5.1 | 47,XY,+21[4]/46,XY,add(6)(q21)[3]/ 46,XY[21] | Positive | — | 0 |
| 9 | 3 | M | 4.7 | 46,XY,add(14)(q32)[11] | Positive | — | — |
| 10 | 1.9 | M | 4.6 | 46,XY,t(1;11)(p32;q23)[14]/46,XY[6] | Positive | — | — |
| 11 | 3.8 | M | 4.3 | 46,XY[20] | Negative | — | 0 |
| 12 | 0.7 | F | 4.3 | — | Negative | — | — |
| 13 | 12 | F | 4.2 | 46,XX,add(5)(q31),t(6;13)(q21;q34)[11]/46,XX[9] | Positive | — | 0.36 |
| 14 | 3 | M | 4.1 | 45,XY,der(12)t(12;13)(p13;q12),-13[4]/46,XY[16]† | Positive | — | 0 |
| 15 | 4.7 | F | 3.9 | 49,XX,+17,+21,+mar[6]/46,XX[10] | Positive | — | 0 |
| 16 | 4.6 | M | 3.7 | 46,XY,add(3)(p14),add(8)(q22),add(12)(p11.1),der(12)t(3;12)(p14;p11.1),i(21)(q10)[17]/46,XY[3]† | Negative | — | 0 |
| 17 | 6 | M | 3.6 | 46,XY[20] | Positive | Exons 4-6 | 0.02 |
| 18 | 18 | F | 3.4 | 46,XY[20] | — | — | 2.7 |
| 19 | 3.8 | M | 3.3 | 46,XY[20] | Negative | — | 0 |
| 20 | 15 | M | 3.3 | 47,XY,+5[20] | Positive | Exons 4-6 | 1.1 |
| 21 | 12 | M | 3.2 | 46,XY[20] | — | — | 0.04 |
| 22 | 3.1 | F | 3 | 46,XX,i(7)(q10),+21c[10]/47,XX,+21c[10](DS) | — | — | 0 |
| 23 | 7 | F | 2.9 | 46,XX[20] | Negative | — | 0 |
| 24 | 16 | M | 2.7 | 46,XY,add(3)(p13),add(5)(q11.2),add(9)(p12),der(9)add(p11)add(q22)[14]/46,XY[4]* | Negative | — | 0 |
| 25 | 2.8 | F | 2.7 | 46,XX,add(12)(p13)[5]/46,XX[15]† | Positive | — | 0 |
| 26‡ | 7 | M | 2.6 | 46,XY,del(5)(q13),der(9)t(5;9)(9qter→9q33::9p21→9q33::5q13→5qter),del(9)(p21)[18]/46,XY[2]* | Positive | — | 0 |
| 27 | 4.1 | M | 2.5 | 45,XY,-8,der(12)t(8;12)(q11.2;p11.2)[10]/46,XY[10]† | Positive | — | 0 |
| 28 | 12 | F | 2.5 | 46,XX,add(1)(p21),add(7)(q11.2),-8,-13,-13, add(16)(q22),−21,+4mar[5]/46,XX[10]§ | Negative | — | 0 |
| 29 | 8 | F | 2.4 | 46,XX[20] | Positive | — | 0 |
| 30 | 1.8 | F | 2.4 | 47,XX,+5,r(9),add(13)(q14)[5]/47,sl,add(7)(q32)[12]/46,XX[3]* | Positive | — | 0 |
| 31 | 3.9 | M | 2.4 | 46,XY[20]¶ | Positive | — | 0 |
| 32|| | 4.4 | M | 2.3 | 46,XY,t(9;22)(q34;q11.2)[1]/46,idem,idic(Y)(p11.3),t(1;2)(p36.1;p12)[16]/46,XY[3]# | Positive | — | — |
| 33 | 3 | M | 2.2 | 46,XY,dic(9;20)(p13;q11.2),+21[20]/46,XY[1]* | Positive | — | 0.09 |
| 34 | 12 | M | 2.2 | 46,XX,r(7),+9[14]/46,XX[6] | Positive | — | 0 |
| 35 | 9 | M | 2.15 | — | — | — | 0 |
| 36 | 1.5 | M | 2 | 60,XY,+X,+4,+6,+8,+10,+11,+12,+14,+14,+17,+18,+21,+21,+22[9]/46,XY[11] | Positive | — | 0 |
F, female; M, male; MRD, minimal residual disease; —, not done.
Fluorescence in situ hybridization (FISH) showed CDKN2A deletion.
FISH showed ETV6/RUNX1 fusion.
Died of relapse 2 years after diagnosis.
FISH showed iAMP21.
Flow cytometry showed hyperdiploidy with DNA index of 1.13; FISH showed extra copies of chromosomes X, 17, 18, 21.
Died 15 days after diagnosis.
FISH showed BCR/ABL1 fusion.