Table 1. Number of LTC-IC–derived CFCs... | American Society of Hematology

Table 1.

Number of LTC-IC–derived CFCs generated at week 5 or week 12 by 1000 2- to 4-division cells sorted on day 4

Experiment	Cell divisions	MS5 cells^*	Weeks in LTC
Experiment	Cell divisions	MS5 cells^*	5	12
CD38^lowSF	2	−	830	278
		+	870	180
	4	−	50	0
		+	90	25
CD38^low+ S	3	−	442	216
		+	1250	910
	4	−	73	1
		+	706	230
CD38^negSF	4	−	133	79
		+	225	102

Experiment	Cell divisions	MS5 cells^*	Weeks in LTC
Experiment	Cell divisions	MS5 cells^*	5	12
CD38^lowSF	2	−	830	278
		+	870	180
	4	−	50	0
		+	90	25
CD38^low+ S	3	−	442	216
		+	1250	910
	4	−	73	1
		+	706	230
CD38^negSF	4	−	133	79
		+	225	102

CD34⁺CD38^low or CD34⁺CD38^neg cells were first exposed to cytokines for 4 days in the presence of serum (S) or in serum-free medium (SF) with or without MS5 cells, as indicated. Cells that had completed 2 or 4 divisions in each condition were then sorted and plated in a standard MS5-based LTC-IC assay and continued for either 5 or 12 weeks.

Numbers refer to the number of CFCs counted after 5 (column 4) or 12 (column 5) weeks in colony assays seeded with the progeny of 1000 cells on day 4. One experiment was performed for each condition.

View Large

This Feature Is Available To Subscribers Only