Table 1. Individual α-granule surface... | American Society of Hematology

Table 1.

Individual α-granule surface area and volume increased upon thrombin stimulation

Time, s	α-Granules
	Surface area per granule, μm²		Volume per granule, μm³
	Condensed	Decondensed	Condensed	Decondensed
0	0.37 ± 0.017	ND	0.017 ± 0.001	ND
0	N = 125	ND	N = 125	ND
90	0.44 ± 0.027	0.85 ± 0.26	0.019 ± 0.001	0.056 ± 0.023
	N = 69	N = 19	N = 69	N = 19
	P = .02		P = .19
300	ND	0.88 ± 0.16	ND	0.055 ± 0.012
		N = 51		N = 51
		P = .05		P = .002

Time, s	α-Granules
	Surface area per granule, μm²		Volume per granule, μm³
	Condensed	Decondensed	Condensed	Decondensed
0	0.37 ± 0.017	ND	0.017 ± 0.001	ND
0	N = 125	ND	N = 125	ND
90	0.44 ± 0.027	0.85 ± 0.26	0.019 ± 0.001	0.056 ± 0.023
	N = 69	N = 19	N = 69	N = 19
	P = .02		P = .19
300	ND	0.88 ± 0.16	ND	0.055 ± 0.012
		N = 51		N = 51
		P = .05		P = .002

WT mouse platelets were stimulated with thrombin for the indicated times. α-Granule surface area and volumes per platelet were determined from full platelet depth images derived from SBF-SEM image stacks. Five platelets were rendered and quantified at each time point (N = total number of condensed or decondensed granules scored at each time point). Bolded numbers were used for P value comparisons. For surface area, significant P values (calculated with Student t test) were seen for 2 comparisons: condensed at 0 vs 90 s (P = .02) and condensed at 90 s vs decondensed at 300 s (P = .05). Volume comparisons showed significant P values for only the condensed at 90 s vs decondensed at 300 s comparison (P = .002).

ND, not detected.

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