Table 1. Mouse mAbs directed against FH... | American Society of Hematology

Table 1.

Mouse mAbs directed against FH used in this study

Designation	Isotype	Epitope location (CCP domain)*	Cross-reactivity	Functional effect†
Anti-FH.02	Mouse IgG1, κ	20	FHR-1	Strong inhibition
Anti-FH.03	Mouse IgG1, κ	Unknown	FHL-1	Weak inhibition
Anti-FH.07	Mouse IgG1, κ	18	FHR-1	Potentiation
Anti-FH.09	Mouse IgG1, κ	6	FHL-1, FHR-3, FHR-4A	Strong inhibition
Anti-FH.10	Mouse IgG1, κ	20	FHR-1	Strong inhibition
Anti-FH.11	Mouse IgG2a, κ	1-4	FHL-1	Strong inhibition‡
Anti-FH.15	Mouse IgG1, κ	5	FHL-1	Strong inhibition
Anti-FH.16	Mouse IgG1, κ	16-17	None	Weak inhibition
Anti-FH.19	Mouse IgG1, κ	20	FHR-1	Strong inhibition

Designation	Isotype	Epitope location (CCP domain)*	Cross-reactivity	Functional effect†
Anti-FH.02	Mouse IgG1, κ	20	FHR-1	Strong inhibition
Anti-FH.03	Mouse IgG1, κ	Unknown	FHL-1	Weak inhibition
Anti-FH.07	Mouse IgG1, κ	18	FHR-1	Potentiation
Anti-FH.09	Mouse IgG1, κ	6	FHL-1, FHR-3, FHR-4A	Strong inhibition
Anti-FH.10	Mouse IgG1, κ	20	FHR-1	Strong inhibition
Anti-FH.11	Mouse IgG2a, κ	1-4	FHL-1	Strong inhibition‡
Anti-FH.15	Mouse IgG1, κ	5	FHL-1	Strong inhibition
Anti-FH.16	Mouse IgG1, κ	16-17	None	Weak inhibition
Anti-FH.19	Mouse IgG1, κ	20	FHR-1	Strong inhibition

Epitope location of each mAb was mapped by determining reactivity to FH fragments (supplemental Figure 2).

Effect of mAbs on FH function was assessed with standard AP activation assays (see Figure 1A-B; supplemental Figure 1).

Anti-FH.11 inhibits the cofactor activity of FH.